3H4T

Chimeric Glycosyltransferase for the generation of novel natural products - GtfAH1 in complex with UDP-2F-Glc

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Glycosyltransferase GtfA, Glycosyltransferase	polymer	404	42107.6	1	UniProt (P96558) UniProt (Q6ZZJ7) Pfam (PF03033) Pfam (PF06722)	Amycolatopsis orientalis	GtfA, orf1, PCZA361.19, GtfA protein
2	B, C, D, E (A)	PHOSPHATE ION	non-polymer		95.0	4	Chemie (PO4)
3	F (A)	URIDINE-5'-DIPHOSPHATE	non-polymer		404.2	1	Chemie (UDP)
4	G (A)	water	water		18.0	589	Chemie (HOH)

Sequence modifications

A: 1 - 214 (UniProt: P96558)

PDB	External Database	Details
Gly 2	Arg 2	SEE REMARK 999
Gln 206	Glu 206	SEE REMARK 999

A: 215 - 390 (UniProt: Q6ZZJ7)

PDB	External Database	Details
Ala 533	-	expression tag
Lys 534	-	expression tag
Leu 535	-	expression tag
Ala 536	-	expression tag
Ala 537	-	expression tag
Ala 538	-	expression tag
Leu 539	-	expression tag
Glu 540	-	expression tag
His 541	-	expression tag
His 542	-	expression tag
His 543	-	expression tag
His 544	-	expression tag
His 545	-	expression tag
His 546	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	42107.6
Non-Polymers*	Number of molecules	5
	Total formula weight	784.0
All*	Total formula weight	42891.6

*Water molecules are not included.