3H4I

Chimeric Glycosyltransferase for the generation of novel natural products

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Glycosyltransferase GtfA, Glycosyltransferase	polymer	404	42107.6	1	UniProt (P96558) UniProt (Q6ZZJ7) Pfam (PF03033) Pfam (PF06722)	Amycolatopsis orientalis	GtfA, orf1, PCZA361.19, GtfA protein
2	B (A)	URIDINE-5'-DIPHOSPHATE-2-DEOXY-2-FLUORO-ALPHA-D-GLUCOSE	non-polymer		568.3	1	Chemie (U2F)
3	C, D (A)	SULFATE ION	non-polymer		96.1	2	Chemie (SO4)
4	E (A)	water	water		18.0	630	Chemie (HOH)

Sequence modifications

A: 1 - 214 (UniProt: P96558)

PDB	External Database	Details
Gly 2	Arg 2	SEE REMARK 999
Gln 206	Glu 206	SEE REMARK 999

A: 215 - 390 (UniProt: Q6ZZJ7)

PDB	External Database	Details
Ala 533	-	expression tag
Lys 534	-	expression tag
Leu 535	-	expression tag
Ala 536	-	expression tag
Ala 537	-	expression tag
Ala 538	-	expression tag
Leu 539	-	expression tag
Glu 540	-	expression tag
His 541	-	expression tag
His 542	-	expression tag
His 543	-	expression tag
His 544	-	expression tag
His 545	-	expression tag
His 546	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	42107.6
Non-Polymers*	Number of molecules	3
	Total formula weight	760.4
All*	Total formula weight	42868.0

*Water molecules are not included.