3H3J
Crystal structure of lactate dehydrogenase mutant (A85R) from staphylococcus aureus complexed with NAD and pyruvate
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | L-lactate dehydrogenase 1 | polymer | 317 | 34707.5 | 2 | UniProt (Q5HJD7) Pfam (PF00056) Pfam (PF02866) | Staphylococcus aureus subsp. aureus COL | L-LDH 1 |
| 2 | C, F (A, B) | NICOTINAMIDE-ADENINE-DINUCLEOTIDE | non-polymer | 663.4 | 2 | Chemie (NAD) | |||
| 3 | D, G (A, B) | PYRUVIC ACID | non-polymer | 88.1 | 2 | Chemie (PYR) | |||
| 4 | E, H (A, B) | GLYCEROL | non-polymer | 92.1 | 2 | Chemie (GOL) | |||
| 5 | I, J (A, B) | water | water | 18.0 | 286 | Chemie (HOH) |
Sequence modifications
A, B: 1 - 317 (UniProt: Q5HJD7)
| PDB | External Database | Details |
|---|---|---|
| Arg 85 | Ala 85 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 69415.0 | |
| Non-Polymers* | Number of molecules | 6 |
| Total formula weight | 1687.2 | |
| All* | Total formula weight | 71102.1 |
