3GUN
T4 lysozyme M102E/L99A mutant with buried charge in apolar cavity--aniline binding
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | Lysozyme | polymer | 164 | 18709.6 | 2 | UniProt (P00720) Pfam (PF00959) | Enterobacteria phage T4 | Lysis protein, Muramidase, Endolysin |
| 2 | C, G, H (A, B) | ANILINE | non-polymer | 93.1 | 3 | Chemie (ANL) | |||
| 3 | D (A) | CALCIUM ION | non-polymer | 40.1 | 1 | Chemie (CA) | |||
| 4 | E, F, I, J (A, B) | CHLORIDE ION | non-polymer | 35.5 | 4 | Chemie (CL) | |||
| 5 | K, L (A, B) | water | water | 18.0 | 219 | Chemie (HOH) |
Sequence modifications
A, B: 1 - 164 (UniProt: P00720)
| PDB | External Database | Details |
|---|---|---|
| Cys 21 | Thr 21 | engineered mutation |
| Asp 38 | Ser 38 | engineered mutation |
| Ala 99 | Leu 99 | engineered mutation |
| Glu 102 | Met 102 | engineered mutation |
| Val 108 | Glu 108 | engineered mutation |
| Val 117 | Ser 117 | engineered mutation |
| Cys 142 | Thr 142 | engineered mutation |
| Asp 144 | Asn 144 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 37419.1 | |
| Non-Polymers* | Number of molecules | 8 |
| Total formula weight | 461.3 | |
| All* | Total formula weight | 37880.4 |
