3GTM

Co-complex of Backtracked RNA polymerase II with TFIIS

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	DNA-directed RNA polymerase II subunit RPB1	polymer	1733	191821.6	1	UniProt (P04050) Pfam (PF04997) Pfam (PF05001) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998) Pfam (PF04992) Pfam (PF04990) In PDB	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	RNA polymerase II subunit B1,DNA-directed RNA polymerase III largest subunit,RNA polymerase II subunit B220
2	B	DNA-directed RNA polymerase II subunit RPB2	polymer	1224	138937.3	1	UniProt (P08518) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF04566) Pfam (PF04567) Pfam (PF00562) Pfam (PF04560) In PDB	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	RNA polymerase II subunit 2,B150,DNA-directed RNA polymerase II 140 kDa polypeptide
3	C	DNA-directed RNA polymerase II subunit RPB3	polymer	318	35330.5	1	UniProt (P16370) Pfam (PF01193) Pfam (PF01000) In PDB	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
4	E	DNA-directed RNA polymerases I, II, and III subunit RPABC1	polymer	215	25117.1	1	UniProt (P20434) Pfam (PF03871) Pfam (PF01191) In PDB	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
5	F	DNA-directed RNA polymerases I, II, and III subunit RPABC2	polymer	155	17931.8	1	UniProt (P20435) Pfam (PF01192) In PDB	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
6	H	DNA-directed RNA polymerases I, II, and III subunit RPABC3	polymer	146	16525.4	1	UniProt (P20436) Pfam (PF03870) In PDB	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	RNA polymerases I,II,and III subunit ABC3,ABC14.4,ABC14.5,DNA-directed RNA polymerases I,and III 14.5 kDa polypeptide
7	I	DNA-directed RNA polymerase II subunit RPB9	polymer	122	14308.2	1	UniProt (P27999) Pfam (PF02150) Pfam (PF01096) In PDB	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	RNA polymerase II subunit B9,B12.6,DNA-directed RNA polymerase II 14.2 kDa polypeptide,DNA-directed RNA polymerase II subunit 9
8	J	DNA-directed RNA polymerases I, II, and III subunit RPABC5	polymer	70	8290.7	1	UniProt (P22139) Pfam (PF01194) In PDB	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	RNA polymerases I,II,and III subunit ABC5,ABC10-beta,ABC8,DNA-directed RNA polymerases I,and III 8.3 kDa polypeptide
9	K	DNA-directed RNA polymerase II subunit RPB11	polymer	120	13633.5	1	UniProt (P38902) Pfam (PF13656) Pfam (PF01193) In PDB	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	RNA polymerase II subunit B11,B13.6,DNA-directed RNA polymerase II 13.6 kDa polypeptide
10	L	DNA-directed RNA polymerases I, II, and III subunit RPABC4	polymer	70	7730.0	1	UniProt (P40422) Pfam (PF03604) In PDB	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	RNA polymerases I,II,and III subunit ABC4,ABC10-alpha
11	S	Transcription elongation factor S-II	polymer	173	19732.4	1	UniProt (P07273) Pfam (PF07500) Pfam (PF01096) In PDB	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	DNA strand transfer protein alpha,STP-alpha,DNA strand transferase 1,Pyrimidine pathway regulatory protein 2
12	M	RNA (5'-R(*AP*UP*CP*GP*AP*GP*AP*GP*GP*AP*UP*GP*C)-3')	polymer	13	4220.6	1		synthetic construct
13	N	DNA (28-MER)	polymer	28	8534.5	1		synthetic construct
14	O	DNA (5'-D(*CP*TP*GP*CP*TP*TP*AP*TP*CP*GP*GP*TP*AP*G)-3')	polymer	14	4286.8	1		synthetic construct
15	A	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)
16	A, B, C, I, J...	ZINC ION	non-polymer		65.4	9	Chemie (ZN)

Sequence modifications

S: 145 - 304 (UniProt: P07273)

PDB	External Database	Details
Met 137	-	initiating methionine
Arg 138	-	expression tag
Gly 139	-	expression tag
Ser 140	-	expression tag
His 141	-	expression tag
His 142	-	expression tag
His 143	-	expression tag
His 144	-	expression tag
His 291	Glu 293	engineered mutation
Arg 305	-	expression tag
Trp 306	-	expression tag
Lys 307	-	expression tag
Phe 308	-	expression tag
Ser 309	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	14
	Total formula weight	506400.3
Non-Polymers*	Number of molecules	10
	Total formula weight	613.0
All*	Total formula weight	507013.3

*Water molecules are not included.