3GTI

D71G/E101G/M234L mutant in organophosphorus hydrolase from Deinococcus radiodurans

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Organophosphorus hydrolase	polymer	337	36295.8	1	UniProt (Q9RVU2) Pfam (PF02126)	Deinococcus radiodurans	Phosphotriesterase, putative
2	B, C (A)	COBALT (II) ION	non-polymer		58.9	2	Chemie (CO)
3	D, E, F (A)	SODIUM ION	non-polymer		23.0	3	Chemie (NA)
4	G (A)	water	water		18.0	327	Chemie (HOH)

Sequence modifications

A: 101 - 423 (UniProt: Q9RVU2)

PDB	External Database	Details
His 87	-	expression tag
His 88	-	expression tag
His 89	-	expression tag
His 90	-	expression tag
His 91	-	expression tag
His 92	-	expression tag
Gly 93	-	expression tag
Leu 94	-	expression tag
Val 95	-	expression tag
Pro 96	-	expression tag
Arg 97	-	expression tag
Gly 98	-	expression tag
Ser 99	-	expression tag
His 100	-	expression tag
Gly 171	Asp 71	engineered mutation
Gly 201	Glu 101	engineered mutation
Leu 334	Met 234	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	36295.8
Non-Polymers*	Number of molecules	5
	Total formula weight	186.8
All*	Total formula weight	36482.6

*Water molecules are not included.