3GTH

D71G/E101G/M234I mutant in organophosphorus hydrolase from Deinococcus radiodurans

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Organophosphorus hydrolase	polymer	337	36153.5	1	UniProt (Q9RVU2) Pfam (PF02126)	Deinococcus radiodurans	Phosphotriesterase, putative
2	B, C, D (A)	FORMIC ACID	non-polymer		46.0	3	Chemie (FMT)
3	E, F (A)	COBALT (II) ION	non-polymer		58.9	2	Chemie (CO)
4	G (A)	water	water		18.0	330	Chemie (HOH)

Sequence modifications

A: 101 - 423 (UniProt: Q9RVU2)

PDB	External Database	Details
His 86	-	expression tag
His 87	-	expression tag
His 88	-	expression tag
His 89	-	expression tag
His 90	-	expression tag
His 91	-	expression tag
Gly 92	-	expression tag
Asp 93	-	expression tag
Ala 94	-	expression tag
Pro 95	-	expression tag
Gly 96	-	expression tag
Gly 97	-	expression tag
Ala 98	-	expression tag
His 99	-	expression tag
Gly 171	Asp 71	engineered mutation
Gly 201	Glu 101	engineered mutation
Ile 334	Met 234	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	36153.5
Non-Polymers*	Number of molecules	5
	Total formula weight	255.9
All*	Total formula weight	36409.5

*Water molecules are not included.