3GOX

Crystal structure of the beta-beta-alpha-Me type II restriction endonuclease Hpy99I in the absence of EDTA

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Restriction endonuclease Hpy99I	polymer	200	22768.2	2	UniProt (Q9ZL26) Pfam (PF18350) Pfam (PF02945)	Helicobacter pylori
2	C (C)	5'-(*DCP*DTP*DCP*DGP*DAP*DCP*DGP*DTP*DAP*DGP*DA)-3'	polymer	11	3358.2	1
3	D (D)	5'-(*DTP*DAP*DCP*DGP*DTP*DCP*DGP*DAP*DGP*DTP*DC)-3'	polymer	11	3349.2	1
4	E, F, I, J (A, B)	ZINC ION	non-polymer		65.4	4	Chemie (ZN)
5	G, K, L (A, B)	SODIUM ION	non-polymer		23.0	3	Chemie (NA)
6	H (A)	PENTAETHYLENE GLYCOL	non-polymer		238.3	1	Chemie (1PE)
7	M, N, O, P (A, B, C, D)	water	water		18.0	476	Chemie (HOH)

Sequence modifications

A, B: 1 - 190 (UniProt: Q9ZL26)

PDB	External Database	Details
Met -9	-	expression tag
Gly -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
Glu -1	-	expression tag
Phe 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	52243.9
Non-Polymers*	Number of molecules	8
	Total formula weight	568.9
All*	Total formula weight	52812.8

*Water molecules are not included.