3GJ7

Crystal structure of human RanGDP-Nup153ZnF12 complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C (A, C)	GTP-binding nuclear protein Ran	polymer	221	24846.5	2	UniProt (P62826) Pfam (PF00071)	Homo sapiens (human)	GTPase Ran, Ras-related nuclear protein, Ras-like protein TC4, Androgen receptor-associated protein 24
2	B, D (B, D)	Nuclear pore complex protein Nup153	polymer	98	10143.6	2	UniProt (P49791) Pfam (PF00641)	Rattus norvegicus (rat)	Nucleoporin Nup153, 153 kDa nucleoporin
3	E, G, H, J, L... (A, C)	MAGNESIUM ION	non-polymer		24.3	6	Chemie (MG) PubChem (888)
4	F, K (A, C)	GUANOSINE-5'-DIPHOSPHATE	non-polymer		443.2	2	Chemie (GDP) PubChem (136073927) PubChem (135398619) PubChem (135402030) PubChem (135431654) PubChem (135566337) PubChem (135760556) PubChem (136026707) PubChem (136053418) PubChem (136663964) PubChem (136664748) PubChem (136759848) PubChem (136759849) PubChem (136759850) PubChem (136836636) PubChem (136847074) PubChem (172898537)
5	I, N (B, D)	ZINC ION	non-polymer		65.4	2	Chemie (ZN)
6	O, P, Q, R (A, B, C, D)	water	water		18.0	294	Chemie (HOH)

Sequence modifications

A, C: 2 - 216 (UniProt: P62826)

PDB	External Database	Details
Gly -4	-	expression tag
Pro -3	-	expression tag
His -2	-	expression tag
Met -1	-	expression tag
Ala 0	-	expression tag
Ser 1	-	expression tag
Ser 35	Phe 35	engineered mutation

B, D: 658 - 750 (UniProt: P49791)

PDB	External Database	Details
Gly 653	-	expression tag
Pro 654	-	expression tag
Leu 655	-	expression tag
Gly 656	-	expression tag
Ser 657	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	69980.1
Non-Polymers*	Number of molecules	10
	Total formula weight	1163.0
All*	Total formula weight	71143.1

*Water molecules are not included.