3GJ7

Crystal structure of human RanGDP-Nup153ZnF12 complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C (A, C)	GTP-binding nuclear protein Ran	polymer	221	24846.5	2	UniProt (P62826) Pfam (PF00071)	Homo sapiens (human)	GTPase Ran, Ras-related nuclear protein, Ras-like protein TC4, Androgen receptor-associated protein 24
2	B, D (B, D)	Nuclear pore complex protein Nup153	polymer	98	10143.6	2	UniProt (P49791) Pfam (PF00641)	Rattus norvegicus (rat)	Nucleoporin Nup153, 153 kDa nucleoporin
3	E, G, H, J, L... (A, C)	MAGNESIUM ION	non-polymer		24.3	6	Chemie (MG)
4	F, K (A, C)	GUANOSINE-5'-DIPHOSPHATE	non-polymer		443.2	2	Chemie (GDP)
5	I, N (B, D)	ZINC ION	non-polymer		65.4	2	Chemie (ZN)
6	O, P, Q, R (A, B, C, D)	water	water		18.0	294	Chemie (HOH)

Sequence modifications

A, C: 2 - 216 (UniProt: P62826)

PDB	External Database	Details
Gly -4	-	expression tag
Pro -3	-	expression tag
His -2	-	expression tag
Met -1	-	expression tag
Ala 0	-	expression tag
Ser 1	-	expression tag
Ser 35	Phe 35	engineered mutation

B, D: 658 - 750 (UniProt: P49791)

PDB	External Database	Details
Gly 653	-	expression tag
Pro 654	-	expression tag
Leu 655	-	expression tag
Gly 656	-	expression tag
Ser 657	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	69980.1
Non-Polymers*	Number of molecules	10
	Total formula weight	1163.0
All*	Total formula weight	71143.1

*Water molecules are not included.