3GJ5

Crystal structure of human RanGDP-Nup153ZnF4 complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C	GTP-binding nuclear protein Ran	polymer	221	24846.5	2	UniProt (P62826) Pfam (PF00071) In PDB	Homo sapiens (human)	GTPase Ran, Ras-related nuclear protein, Ras-like protein TC4, Androgen receptor-associated protein 24
2	B, D	Nuclear pore complex protein Nup153	polymer	34	3458.9	2	UniProt (P49791) Pfam (PF00641) In PDB	Rattus norvegicus (rat)	Nucleoporin Nup153, 153 kDa nucleoporin
3	A, C	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
4	A, C	GUANOSINE-5'-DIPHOSPHATE	non-polymer		443.2	2	Chemie (GDP)
5	B, D	ZINC ION	non-polymer		65.4	2	Chemie (ZN)
6		water	water		18.0	458	Chemie (HOH)

Sequence modifications

A, C: 2 - 216 (UniProt: P62826)

PDB	External Database	Details
Gly -4	-	expression tag
Pro -3	-	expression tag
His -2	-	expression tag
Met -1	-	expression tag
Ala 0	-	expression tag
Ser 1	-	expression tag
Ser 35	Phe 35	engineered mutation

B, D: 848 - 876 (UniProt: P49791)

PDB	External Database	Details
Gly 843	-	expression tag
Pro 844	-	expression tag
Leu 845	-	expression tag
Gly 846	-	expression tag
Ser 847	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	56610.7
Non-Polymers*	Number of molecules	6
	Total formula weight	1065.8
All*	Total formula weight	57676.6

*Water molecules are not included.