3GHN

Crystal structure of the exosite-containing fragment of human ADAMTS13 (form-2)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	A disintegrin and metalloproteinase with thrombospondin motifs 13	polymer	419	46527.5	1	UniProt (Q76LX8) Pfam (PF17771) Pfam (PF00090) Pfam (PF19236) Pfam (PF05986)	Homo sapiens (human)	ADAMTS-13, ADAM-TS 13, ADAM-TS13, von Willebrand factor-cleaving protease, vWF-cleaving protease, vWF-CP
2	B (B)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	1	In PDB GlyTouCan (G42666HT)
3	C (C)	beta-D-glucopyranose-(1-3)-alpha-L-fucopyranose	branched		326.3	1	In PDB GlyTouCan (G36855WW)
4	D, E (A)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	2	Chemie (NAG)
5	F (A)	water	water		18.0	47	Chemie (HOH)

Sequence modifications

A: 287 - 685 (UniProt: Q76LX8)

PDB	External Database	Details
Gly 285	-	expression tag
Arg 286	-	expression tag
Leu 524	Ser 524	engineered mutation
Ser 686	-	expression tag
Arg 687	-	expression tag
Leu 688	-	expression tag
Glu 689	-	expression tag
Asn 690	-	expression tag
Leu 691	-	expression tag
Tyr 692	-	expression tag
Phe 693	-	expression tag
Gln 694	-	expression tag
Ser 695	-	expression tag
Arg 696	-	expression tag
Leu 697	-	expression tag
Glu 698	-	expression tag
Asn 699	-	expression tag
Leu 700	-	expression tag
Tyr 701	-	expression tag
Phe 702	-	expression tag
Gln 703	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	46527.5
Branched	Number of molecules	2
	Total formula weight	750.7
Non-Polymers*	Number of molecules	2
	Total formula weight	442.4
All*	Total formula weight	47720.7

*Water molecules are not included.