3GGS

Human purine nucleoside phosphorylase double mutant E201Q,N243D complexed with 2-fluoro-2'-deoxyadenosine

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C	Purine nucleoside phosphorylase	polymer	311	34400.2	3	UniProt (P00491) Pfam (PF01048) In PDB	Homo sapiens (Human)	PNP, Inosine phosphorylase
2	A, C, B	5-(6-AMINO-2-FLUORO-PURIN-9-YL)-2-HYDROXYMETHYL-TETRAHYDRO-FURAN-3-OL	non-polymer		269.2	4	Chemie (2FD)
3	C, A, B	SULFATE ION	non-polymer		96.1	30	Chemie (SO4)
4		water	water		18.0	64	Chemie (HOH)

Sequence modifications

A, B, C: 1 - 289 (UniProt: P00491)

PDB	External Database	Details
Lys -21	-	expression tag
Glu -20	-	expression tag
Thr -19	-	expression tag
Ala -18	-	expression tag
Ala -17	-	expression tag
Ala -16	-	expression tag
Lys -15	-	expression tag
Phe -14	-	expression tag
Glu -13	-	expression tag
Arg -12	-	expression tag
Gln -11	-	expression tag
His -10	-	expression tag
Met -9	-	expression tag
Asp -8	-	expression tag
Ser -7	-	expression tag
Gly -6	-	expression tag
Gly -5	-	expression tag
Gly -4	-	expression tag
Gly -3	-	expression tag
Ser -2	-	expression tag
Gly -1	-	expression tag
His 0	-	expression tag
Gln 201	Glu 201	engineered mutation
Asp 243	Asn 243	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	103200.7
Non-Polymers*	Number of molecules	34
	Total formula weight	3958.8
All*	Total formula weight	107159.6

*Water molecules are not included.