3G8V
The rationally designed catalytically inactive mutant Mth0212(D151N)
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Exodeoxyribonuclease | polymer | 265 | 31429.7 | 1 | UniProt (O26314) Pfam (PF03372) | Methanothermobacter thermautotrophicus | Mth0212 |
| 2 | B, C, D (A) | GLYCEROL | non-polymer | 92.1 | 3 | Chemie (GOL) | |||
| 3 | E (A) | TETRAETHYLENE GLYCOL | non-polymer | 194.2 | 1 | Chemie (PG4) | |||
| 4 | F (A) | water | water | 18.0 | 100 | Chemie (HOH) |
Sequence modifications
A: 1 - 257 (UniProt: O26314)
| PDB | External Database | Details |
|---|---|---|
| Ala 2 | Thr 2 | engineered mutation |
| Asn 151 | Asp 151 | engineered mutation |
| Leu 258 | - | expression tag |
| Glu 259 | - | expression tag |
| His 260 | - | expression tag |
| His 261 | - | expression tag |
| His 262 | - | expression tag |
| His 263 | - | expression tag |
| His 264 | - | expression tag |
| His 265 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 31429.7 | |
| Non-Polymers* | Number of molecules | 4 |
| Total formula weight | 470.5 | |
| All* | Total formula weight | 31900.2 |
