3G8V

The rationally designed catalytically inactive mutant Mth0212(D151N)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Exodeoxyribonuclease	polymer	265	31429.7	1	UniProt (O26314) Pfam (PF03372) In PDB	Methanothermobacter thermautotrophicus	Mth0212
2	A	GLYCEROL	non-polymer		92.1	3	Chemie (GOL)
3	A	TETRAETHYLENE GLYCOL	non-polymer		194.2	1	Chemie (PG4)
4		water	water		18.0	100	Chemie (HOH)

Sequence modifications

A: 1 - 257 (UniProt: O26314)

PDB	External Database	Details
Ala 2	Thr 2	engineered mutation
Asn 151	Asp 151	engineered mutation
Leu 258	-	expression tag
Glu 259	-	expression tag
His 260	-	expression tag
His 261	-	expression tag
His 262	-	expression tag
His 263	-	expression tag
His 264	-	expression tag
His 265	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	31429.7
Non-Polymers*	Number of molecules	4
	Total formula weight	470.5
All*	Total formula weight	31900.2

*Water molecules are not included.