3G3J

Crystal structure of the GluR6 ligand binding domain dimer I442H K494E K665R I749L Q753K mutant with glutamate and NaCl at 1.32 Angstrom resolution

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Glutamate receptor, ionotropic kainate 2	polymer	259	29425.6	2	UniProt (P42260) Pfam (PF10613) Pfam (PF00060)	Rattus norvegicus	Glutamate receptor 6, GluR-6, GluR6
2	C, H (A, B)	GLUTAMIC ACID	non-polymer		147.1	2	Chemie (GLU)
3	D, E (A)	CHLORIDE ION	non-polymer		35.5	2	Chemie (CL)
4	F, G, I, J (A, B)	SODIUM ION	non-polymer		23.0	4	Chemie (NA)
5	K, L (A, B)	water	water		18.0	618	Chemie (HOH)

Sequence modifications

A, B: 2 - 117 (UniProt: P42260)

PDB	External Database	Details
Gly 1	-	expression tag
His 46	Ile 473	engineered mutation
Glu 98	Lys 525	engineered mutation

A, B: 120 - 259 (UniProt: P42260)

PDB	External Database	Details
Gly 118	-	linker
Thr 119	-	linker
Arg 149	Lys 696	engineered mutation
Leu 233	Ile 780	engineered mutation
Lys 237	Gln 784	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	58851.2
Non-Polymers*	Number of molecules	8
	Total formula weight	457.1
All*	Total formula weight	59308.4

*Water molecules are not included.