3FPJ
Crystal Structure of E81Q mutant of MtNAS in complex with S-ADENOSYLMETHIONINE
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, B | Putative uncharacterized protein | polymer | 298 | 33849.7 | 2 | UniProt (O26771) Pfam (PF03059) In PDB | Methanothermobacter thermautotrophicus | MtNAS |
2 | A, B | S-ADENOSYLMETHIONINE | non-polymer | 398.4 | 2 | Chemie (SAM) | |||
3 | A, B | BROMIDE ION | non-polymer | 79.9 | 3 | Chemie (BR) | |||
4 | B | 2-[3-(2-HYDROXY-1,1-DIHYDROXYMETHYL-ETHYLAMINO)-PROPYLAMINO]-2-HYDROXYMETHYL-PROPANE-1,3-DIOL | non-polymer | 282.3 | 1 | Chemie (B3P) | |||
5 | water | water | 18.0 | 540 | Chemie (HOH) |
Sequence modifications
A, B: 1 - 266 (UniProt: O26771)
PDB | External Database | Details |
---|---|---|
Gln 81 | Glu 81 | engineered mutation |
Lys 267 | - | expression tag |
Gly 268 | - | expression tag |
Glu 269 | - | expression tag |
Leu 270 | - | expression tag |
Asn 271 | - | expression tag |
Ser 272 | - | expression tag |
Lys 273 | - | expression tag |
Leu 274 | - | expression tag |
Glu 275 | - | expression tag |
Gly 276 | - | expression tag |
Lys 277 | - | expression tag |
Pro 278 | - | expression tag |
Ile 279 | - | expression tag |
Pro 280 | - | expression tag |
Asn 281 | - | expression tag |
Pro 282 | - | expression tag |
Leu 283 | - | expression tag |
Leu 284 | - | expression tag |
Gly 285 | - | expression tag |
Leu 286 | - | expression tag |
Asp 287 | - | expression tag |
Ser 288 | - | expression tag |
Thr 289 | - | expression tag |
Arg 290 | - | expression tag |
Thr 291 | - | expression tag |
Gly 292 | - | expression tag |
His 293 | - | expression tag |
His 294 | - | expression tag |
His 295 | - | expression tag |
His 296 | - | expression tag |
His 297 | - | expression tag |
His 298 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 2 |
Total formula weight | 67699.5 | |
Non-Polymers* | Number of molecules | 6 |
Total formula weight | 1318.9 | |
All* | Total formula weight | 69018.4 |