3FPH

Crystal Structure of E81Q mutant of MtNAS in complex with L-Glutamate

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Putative uncharacterized protein	polymer	298	33849.7	2	UniProt (O26771) Pfam (PF03059)	Methanothermobacter thermautotrophicus	MtNAS
2	C, E (A, B)	GLUTAMIC ACID	non-polymer		147.1	2	Chemie (GLU)
3	D, F (A, B)	SODIUM ION	non-polymer		23.0	2	Chemie (NA)
4	G, H (A, B)	water	water		18.0	713	Chemie (HOH)

Sequence modifications

A, B: 1 - 266 (UniProt: O26771)

PDB	External Database	Details
Gln 81	Glu 81	engineered mutation
Lys 267	-	expression tag
Gly 268	-	expression tag
Glu 269	-	expression tag
Leu 270	-	expression tag
Asn 271	-	expression tag
Ser 272	-	expression tag
Lys 273	-	expression tag
Leu 274	-	expression tag
Glu 275	-	expression tag
Gly 276	-	expression tag
Lys 277	-	expression tag
Pro 278	-	expression tag
Ile 279	-	expression tag
Pro 280	-	expression tag
Asn 281	-	expression tag
Pro 282	-	expression tag
Leu 283	-	expression tag
Leu 284	-	expression tag
Gly 285	-	expression tag
Leu 286	-	expression tag
Asp 287	-	expression tag
Ser 288	-	expression tag
Thr 289	-	expression tag
Arg 290	-	expression tag
Thr 291	-	expression tag
Gly 292	-	expression tag
His 293	-	expression tag
His 294	-	expression tag
His 295	-	expression tag
His 296	-	expression tag
His 297	-	expression tag
His 298	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	67699.5
Non-Polymers*	Number of molecules	4
	Total formula weight	340.2
All*	Total formula weight	68039.7

*Water molecules are not included.