3FIL
Structural and energetic determinants for hyperstable variants of GB1 obtained from in-vitro evolution
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Immunoglobulin G-binding protein G | polymer | 56 | 6250.0 | 1 | UniProt (P19909) Pfam (PF01378) | Streptococcus sp. 'group G' | IgG-binding protein G |
| 2 | B (B) | Immunoglobulin G-binding protein G | polymer | 56 | 6222.0 | 1 | UniProt (P19909) Pfam (PF01378) | Streptococcus sp. 'group G' | IgG-binding protein G |
| 3 | C (A) | CALCIUM ION | non-polymer | 40.1 | 1 | Chemie (CA) | |||
| 4 | D, E (A, B) | water | water | 18.0 | 239 | Chemie (HOH) |
Sequence modifications
A: 2 - 56 (UniProt: P19909)
B: 2 - 56 (UniProt: P19909)
| PDB | External Database | Details |
|---|---|---|
| Fme 1 | - | SEE REMARK 999 |
| Gln 2 | Thr 303 | engineered mutation |
| Val 15 | Glu 316 | engineered mutation |
| Leu 16 | Thr 317 | engineered mutation |
| Ile 18 | Thr 319 | engineered mutation |
| Leu 37 | Asn 338 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Met 1 | - | initiating methionine |
| Gln 2 | Thr 303 | engineered mutation |
| Val 15 | Glu 316 | engineered mutation |
| Leu 16 | Thr 317 | engineered mutation |
| Ile 18 | Thr 319 | engineered mutation |
| Leu 37 | Asn 338 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 12472.0 | |
| Non-Polymers* | Number of molecules | 1 |
| Total formula weight | 40.1 | |
| All* | Total formula weight | 12512.1 |
