3FGZ

Crystal Structure of CheY triple mutant F14E, N59M, E89R complexed with BeF3- and Mn2+

Replaces:  2J8E

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Chemotaxis protein cheY	polymer	128	14008.2	2	UniProt (P0AE67) Pfam (PF00072)	Escherichia coli
2	C, F (A, B)	MANGANESE (II) ION	non-polymer		54.9	2	Chemie (MN)
3	D, G (A, B)	BERYLLIUM TRIFLUORIDE ION	non-polymer		66.0	2	Chemie (BEF)
4	E, H (A, B)	GLYCEROL	non-polymer		92.1	2	Chemie (GOL)
5	I (B)	AMMONIUM ION	non-polymer		18.0	1	Chemie (NH4)
6	J, K (A, B)	water	water		18.0	169	Chemie (HOH)

Sequence modifications

A, B: 2 - 129 (UniProt: P0AE67)

PDB	External Database	Details
Glu 14	Phe 14	engineered mutation
Met 59	Asn 59	engineered mutation
Arg 89	Glu 89	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	28016.5
Non-Polymers*	Number of molecules	7
	Total formula weight	444.1
All*	Total formula weight	28460.6

*Water molecules are not included.