3FDS

Structural insight into recruitment of translesion DNA polymerase Dpo4 to sliding clamp PCNA

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	DNA polymerase IV	polymer	352	40257.9	1	UniProt (Q97W02) Pfam (PF00817) Pfam (PF11798) Pfam (PF11799) In PDB	Sulfolobus solfataricus	Pol IV
2	C	DNA polymerase sliding clamp B	polymer	249	27521.7	1	UniProt (P57766) Pfam (PF00705) In PDB	Sulfolobus solfataricus	Proliferating cell nuclear antigen homolog B, PCNA B
3	D	DNA polymerase sliding clamp C	polymer	245	27477.2	1	UniProt (Q97Z84) Pfam (PF00705) Pfam (PF02747) In PDB	Sulfolobus solfataricus	Proliferating cell nuclear antigen homolog C, PCNA C
4	C, A, D	1,2-ETHANEDIOL	non-polymer		62.1	26	Chemie (EDO)
5	C, A	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	2	Chemie (PEG)
6	A	PENTAETHYLENE GLYCOL	non-polymer		238.3	1	Chemie (1PE)
7	C, A	GLYCEROL	non-polymer		92.1	3	Chemie (GOL)
8	D	TRIETHYLENE GLYCOL	non-polymer		150.2	1	Chemie (PGE)
9		water	water		18.0	317	Chemie (HOH)

Sequence modifications

C: 1 - 249 (UniProt: P57766)

PDB	External Database	Details
Val 2	Phe 2	engineered mutation

D: 2 - 245 (UniProt: Q97Z84)

PDB	External Database	Details
Met 1	-	initiating methionine

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	95256.8
Non-Polymers*	Number of molecules	33
	Total formula weight	2490.7
All*	Total formula weight	97747.5

*Water molecules are not included.