3FBN

Structure of the Mediator submodule Med7N/31

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C (A, C)	Mediator of RNA polymerase II transcription subunit 7	polymer	84	9286.3	2	UniProt (Q08278) Pfam (PF05983)	Saccharomyces cerevisiae (Baker's yeast)	Mediator complex subunit 7
2	B, D (B, D)	Mediator of RNA polymerase II transcription subunit 31	polymer	130	15030.0	2	UniProt (P38633) Pfam (PF05669)	Saccharomyces cerevisiae (Baker's yeast)	Mediator complex subunit 31, Hpr1 suppressor protein 1
3	E, F, G (A, B, D)	water	water		18.0	5	Chemie (HOH)

Sequence modifications

A, C: 3 - 84 (UniProt: Q08278)

PDB	External Database	Details
Met 1	-	expression tag
Ala 2	-	expression tag
Tyr 13	Leu 12	engineered mutation
Ser 14	Tyr 13	engineered mutation
Thr 16	Pro 15	engineered mutation
Ser 17	Pro 16	engineered mutation
Ser 19	Pro 18	engineered mutation

B, D: 4 - 130 (UniProt: P38633)

PDB	External Database	Details
Gly 1	-	expression tag
Ser 2	-	expression tag
His 3	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	48632.6
All*	Total formula weight	48632.6

*Water molecules are not included.