3F5K

Semi-active E176Q mutant of rice BGlu1, a plant exoglucanase/beta-glucosidase

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Beta-glucosidase	polymer	481	54727.5	2	UniProt (Q42975) Pfam (PF00232) UniProt (by SIFTS) (Q75I93) In PDB	Oryza sativa Japonica Group (Japanese rice)
2	C, D	beta-D-glucopyranose-(1-4)-beta-D-glucopyranose-(1-4)-beta-D-glucopyranose-(1-4)-beta-D-glucopyranose-(1-4)-beta-D-glucopyranose	branched		828.7	2	In PDB BIRD (PRD_900016) GlyTouCan (G91683DU)		beta-cellopentaose
3	A	ZINC ION	non-polymer		65.4	1	Chemie (ZN)
4	A, B	SULFATE ION	non-polymer		96.1	2	Chemie (SO4)
5	A, B	2-(N-MORPHOLINO)-ETHANESULFONIC ACID	non-polymer		195.2	2	Chemie (MES)
6	A, B	GLYCEROL	non-polymer		92.1	2	Chemie (GOL)
7		water	water		18.0	801	Chemie (HOH)

Sequence modifications

A, B: 1 - 476 (UniProt: Q42975)

PDB	External Database	Details
Ala -4	-	expression tag
Met -3	-	expression tag
Ala -2	-	expression tag
Asp -1	-	expression tag
Val 0	-	expression tag
Gln 176	Glu 204	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	109455.0
Branched	Number of molecules	2
	Total formula weight	1657.4
Non-Polymers*	Number of molecules	7
	Total formula weight	832.2
All*	Total formula weight	111944.6

*Water molecules are not included.