3F49
Anion-triggered Engineered Subtilisin SUBT_BACAM
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (S) | Subtilisin BPN | polymer | 266 | 26436.4 | 1 | UniProt (P00782) Pfam (PF00082) | Bacillus amyloliquefaciens | Subtilisin Novo, Subtilisin DFE, Alkaline protease |
| 2 | B (S) | SODIUM ION | non-polymer | 23.0 | 1 | Chemie (NA) | |||
| 3 | C (S) | water | water | 18.0 | 205 | Chemie (HOH) |
Sequence modifications
S: 1 - 275 (UniProt: P00782)
| PDB | External Database | Details |
|---|---|---|
| Lys 2 | Gln 109 | engineered mutation |
| Cys 3 | Ser 110 | engineered mutation |
| Ser 5 | Pro 112 | engineered mutation |
| Ala 9 | Ser 116 | engineered mutation |
| Leu 31 | Ile 138 | engineered mutation |
| Ala 32 | Asp 139 | engineered mutation |
| Asn 43 | Lys 150 | engineered mutation |
| Phe 50 | Met 157 | engineered mutation |
| Leu 73 | Ala 180 | engineered mutation |
| - | Leu 182 | deletion |
| - | Asn 183 | deletion |
| - | Asn 184 | deletion |
| - | Ser 185 | deletion |
| - | Ile 186 | deletion |
| - | Gly 187 | deletion |
| - | Val 188 | deletion |
| - | Leu 189 | deletion |
| - | Gly 190 | deletion |
| Ala 104 | Tyr 211 | engineered mutation |
| Ser 128 | Gly 235 | engineered mutation |
| Ser 156 | Glu 263 | engineered mutation |
| Ser 166 | Gly 273 | engineered mutation |
| Ala 169 | Gly 276 | engineered mutation |
| Pro 188 | Ser 295 | engineered mutation |
| Cys 206 | Gln 313 | engineered mutation |
| Gly 212 | Asn 319 | engineered mutation |
| Leu 217 | Tyr 324 | engineered mutation |
| Ser 218 | Asn 325 | engineered mutation |
| Ala 254 | Thr 361 | engineered mutation |
| Glu 271 | Gln 378 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 26436.4 | |
| Non-Polymers* | Number of molecules | 1 |
| Total formula weight | 23.0 | |
| All* | Total formula weight | 26459.4 |
