3EZW
Crystal Structure of a Hyperactive Escherichia coli Glycerol Kinase Mutant Gly230 --> Asp Obtained Using Microfluidic Crystallization Devices
Replaces: 2P3REntity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, B, E, G, C... | Glycerol kinase | polymer | 526 | 59204.6 | 8 | UniProt (P0A6F3) Pfam (PF00370) Pfam (PF02782) In PDB | Escherichia coli | ATP:glycerol 3-phosphotransferase, Glycerokinase, GK |
2 | C, D, A, F, B... | GLYCEROL | non-polymer | 92.1 | 8 | Chemie (GOL) | |||
3 | C, D, A, F, B... | CHLORIDE ION | non-polymer | 35.5 | 8 | Chemie (CL) | |||
4 | A | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) | |||
5 | C, D, A, F, B... | 1,2-ETHANEDIOL | non-polymer | 62.1 | 19 | Chemie (EDO) | |||
6 | water | water | 18.0 | 1526 | Chemie (HOH) |
Sequence modifications
A, B, E, G, C, D, F, H: 1 - 500 (UniProt: P0A6F3)
PDB | External Database | Details |
---|---|---|
Asp 230 | Gly 231 | engineered mutation |
Gln 501 | - | expression tag |
Leu 502 | - | expression tag |
Tyr 503 | - | expression tag |
Thr 504 | - | expression tag |
Arg 505 | - | expression tag |
Ala 506 | - | expression tag |
Ser 507 | - | expression tag |
Gln 508 | - | expression tag |
Pro 509 | - | expression tag |
Glu 510 | - | expression tag |
Leu 511 | - | expression tag |
Ala 512 | - | expression tag |
Pro 513 | - | expression tag |
Glu 514 | - | expression tag |
Asp 515 | - | expression tag |
Pro 516 | - | expression tag |
Glu 517 | - | expression tag |
Asp 518 | - | expression tag |
Leu 519 | - | expression tag |
Glu 520 | - | expression tag |
His 521 | - | expression tag |
His 522 | - | expression tag |
His 523 | - | expression tag |
His 524 | - | expression tag |
His 525 | - | expression tag |
His 526 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 8 |
Total formula weight | 473636.8 | |
Non-Polymers* | Number of molecules | 36 |
Total formula weight | 2224.0 | |
All* | Total formula weight | 475860.8 |