3EZW

Crystal Structure of a Hyperactive Escherichia coli Glycerol Kinase Mutant Gly230 --> Asp Obtained Using Microfluidic Crystallization Devices

Replaces:  2P3R

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D, E... (A, B, E, G, C...)	Glycerol kinase	polymer	526	59204.6	8	UniProt (P0A6F3) Pfam (PF00370) Pfam (PF02782)	Escherichia coli	ATP:glycerol 3-phosphotransferase, Glycerokinase, GK
2	AA, GA, I, KA, N... (C, D, A, F, B...)	GLYCEROL	non-polymer		92.1	8	Chemie (GOL)
3	BA, HA, J, LA, O... (C, D, A, F, B...)	CHLORIDE ION	non-polymer		35.5	8	Chemie (CL)
4	K (A)	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)
5	CA, DA, EA, FA, IA... (C, D, A, F, B...)	1,2-ETHANEDIOL	non-polymer		62.1	19	Chemie (EDO)
6	SA, TA, UA, VA, WA... (A, B, E, G, C...)	water	water		18.0	1526	Chemie (HOH)

Sequence modifications

A, B, E, G, C, D, F, H: 1 - 500 (UniProt: P0A6F3)

PDB	External Database	Details
Asp 230	Gly 231	engineered mutation
Gln 501	-	expression tag
Leu 502	-	expression tag
Tyr 503	-	expression tag
Thr 504	-	expression tag
Arg 505	-	expression tag
Ala 506	-	expression tag
Ser 507	-	expression tag
Gln 508	-	expression tag
Pro 509	-	expression tag
Glu 510	-	expression tag
Leu 511	-	expression tag
Ala 512	-	expression tag
Pro 513	-	expression tag
Glu 514	-	expression tag
Asp 515	-	expression tag
Pro 516	-	expression tag
Glu 517	-	expression tag
Asp 518	-	expression tag
Leu 519	-	expression tag
Glu 520	-	expression tag
His 521	-	expression tag
His 522	-	expression tag
His 523	-	expression tag
His 524	-	expression tag
His 525	-	expression tag
His 526	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	473636.8
Non-Polymers*	Number of molecules	36
	Total formula weight	2224.0
All*	Total formula weight	475860.8

*Water molecules are not included.