3EXI

Crystal structure of the pyruvate dehydrogenase (E1p) component of human pyruvate dehydrogenase complex with the subunit-binding domain (SBD) of E2p, but SBD cannot be modeled into the electron density

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial	polymer	382	42568.5	1	UniProt (P08559) Pfam (PF00676) In PDB	Homo sapiens (human)	pyruvate dehydrogenase (E1p) alpha subunit; PDHE1-A type I
2	B	Pyruvate dehydrogenase E1 component subunit beta, mitochondrial	polymer	329	35941.3	1	UniProt (P11177) Pfam (PF02779) Pfam (PF02780) In PDB	Homo sapiens (human)	pyruvate dehydrogenase (E1p) beta subunit; PDHE1-B
3	A, B	POTASSIUM ION	non-polymer		39.1	2	Chemie (K)
4	A	CHLORIDE ION	non-polymer		35.5	1	Chemie (CL)
5		water	water		18.0	318	Chemie (HOH)

Sequence modifications

A: 1 - 361 (UniProt: P08559)

PDB	External Database	Details
Met -20	-	expression tag
Gly -19	-	expression tag
Ser -18	-	expression tag
Ser -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
Ser -10	-	expression tag
Ser -9	-	expression tag
Gly -8	-	expression tag
Leu -7	-	expression tag
Val -6	-	expression tag
Pro -5	-	expression tag
Arg -4	-	expression tag
Gly -3	-	expression tag
Ser -2	-	expression tag
His -1	-	expression tag
Met 0	-	expression tag
Phe 89	Tyr 118	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	78509.8
Non-Polymers*	Number of molecules	3
	Total formula weight	113.6
All*	Total formula weight	78623.5

*Water molecules are not included.