3EXH

Crystal structure of the pyruvate dehydrogenase (E1p) component of human pyruvate dehydrogenase complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, E (A, E)	Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial	polymer	382	42552.5	2	UniProt (P08559) Pfam (PF00676)	Homo sapiens (human)	pyruvate dehydrogenase (E1p) alpha subunit; PDHE1-A type I
2	B, D, F, H (B, D, F, H)	Pyruvate dehydrogenase E1 component subunit beta, mitochondrial	polymer	329	35941.3	4	UniProt (P11177) Pfam (PF02779) Pfam (PF02780)	Homo sapiens (human)	pyruvate dehydrogenase (E1p) beta subunit; PDHE1-B
3	C, G (C, G)	Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial	polymer	382	42632.5	2	UniProt (P08559) Pfam (PF00676)	Homo sapiens (human)	pyruvate dehydrogenase (E1p) alpha subunit; PDHE1-A type I
4	I, N, R, V (A, C, E, G)	MANGANESE (II) ION	non-polymer		54.9	4	Chemie (MN)
5	J, O, S, W (A, C, E, G)	POTASSIUM ION	non-polymer		39.1	4	Chemie (K)
6	K, P, T, X (A, C, E, G)	THIAMINE DIPHOSPHATE	non-polymer		425.3	4	Chemie (TPP)
7	L, M, Q, U, Y (A, C, E, G)	GLYCEROL	non-polymer		92.1	5	Chemie (GOL)
8	AA, BA, CA, DA, EA... (B, C, D, E, F...)	water	water		18.0	792	Chemie (HOH)

Sequence modifications

A, E: 1 - 361 (UniProt: P08559)

PDB	External Database	Details
Met -20	-	expression tag
Gly -19	-	expression tag
Ser -18	-	expression tag
Ser -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
Ser -10	-	expression tag
Ser -9	-	expression tag
Gly -8	-	expression tag
Leu -7	-	expression tag
Val -6	-	expression tag
Pro -5	-	expression tag
Arg -4	-	expression tag
Gly -3	-	expression tag
Ser -2	-	expression tag
His -1	-	expression tag
Met 0	-	expression tag
Ala 203	Ser 232	engineered mutation
Ala 271	Ser 300	engineered mutation

C, G: 1 - 361 (UniProt: P08559)

PDB	External Database	Details
Met -20	-	expression tag
Gly -19	-	expression tag
Ser -18	-	expression tag
Ser -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
Ser -10	-	expression tag
Ser -9	-	expression tag
Gly -8	-	expression tag
Leu -7	-	expression tag
Val -6	-	expression tag
Pro -5	-	expression tag
Arg -4	-	expression tag
Gly -3	-	expression tag
Ser -2	-	expression tag
His -1	-	expression tag
Met 0	-	expression tag
Ala 203	Ser 232	engineered mutation
Ala 271	Ser 300	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	314135.3
Non-Polymers*	Number of molecules	17
	Total formula weight	2537.9
All*	Total formula weight	316673.2

*Water molecules are not included.