3EXH
Crystal structure of the pyruvate dehydrogenase (E1p) component of human pyruvate dehydrogenase complex
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, E (A, E) | Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial | polymer | 382 | 42552.5 | 2 | UniProt (P08559) Pfam (PF00676) | Homo sapiens (human) | pyruvate dehydrogenase (E1p) alpha subunit; PDHE1-A type I |
| 2 | B, D, F, H (B, D, F, H) | Pyruvate dehydrogenase E1 component subunit beta, mitochondrial | polymer | 329 | 35941.3 | 4 | UniProt (P11177) Pfam (PF02779) Pfam (PF02780) | Homo sapiens (human) | pyruvate dehydrogenase (E1p) beta subunit; PDHE1-B |
| 3 | C, G (C, G) | Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial | polymer | 382 | 42632.5 | 2 | UniProt (P08559) Pfam (PF00676) | Homo sapiens (human) | pyruvate dehydrogenase (E1p) alpha subunit; PDHE1-A type I |
| 4 | I, N, R, V (A, C, E, G) | MANGANESE (II) ION | non-polymer | 54.9 | 4 | Chemie (MN) | |||
| 5 | J, O, S, W (A, C, E, G) | POTASSIUM ION | non-polymer | 39.1 | 4 | Chemie (K) | |||
| 6 | K, P, T, X (A, C, E, G) | THIAMINE DIPHOSPHATE | non-polymer | 425.3 | 4 | Chemie (TPP) | |||
| 7 | L, M, Q, U, Y (A, C, E, G) | GLYCEROL | non-polymer | 92.1 | 5 | Chemie (GOL) | |||
| 8 | AA, BA, CA, DA, EA... (B, C, D, E, F...) | water | water | 18.0 | 792 | Chemie (HOH) |
Sequence modifications
A, E: 1 - 361 (UniProt: P08559)
C, G: 1 - 361 (UniProt: P08559)
| PDB | External Database | Details |
|---|---|---|
| Met -20 | - | expression tag |
| Gly -19 | - | expression tag |
| Ser -18 | - | expression tag |
| Ser -17 | - | expression tag |
| His -16 | - | expression tag |
| His -15 | - | expression tag |
| His -14 | - | expression tag |
| His -13 | - | expression tag |
| His -12 | - | expression tag |
| His -11 | - | expression tag |
| Ser -10 | - | expression tag |
| Ser -9 | - | expression tag |
| Gly -8 | - | expression tag |
| Leu -7 | - | expression tag |
| Val -6 | - | expression tag |
| Pro -5 | - | expression tag |
| Arg -4 | - | expression tag |
| Gly -3 | - | expression tag |
| Ser -2 | - | expression tag |
| His -1 | - | expression tag |
| Met 0 | - | expression tag |
| Ala 203 | Ser 232 | engineered mutation |
| Ala 271 | Ser 300 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Met -20 | - | expression tag |
| Gly -19 | - | expression tag |
| Ser -18 | - | expression tag |
| Ser -17 | - | expression tag |
| His -16 | - | expression tag |
| His -15 | - | expression tag |
| His -14 | - | expression tag |
| His -13 | - | expression tag |
| His -12 | - | expression tag |
| His -11 | - | expression tag |
| Ser -10 | - | expression tag |
| Ser -9 | - | expression tag |
| Gly -8 | - | expression tag |
| Leu -7 | - | expression tag |
| Val -6 | - | expression tag |
| Pro -5 | - | expression tag |
| Arg -4 | - | expression tag |
| Gly -3 | - | expression tag |
| Ser -2 | - | expression tag |
| His -1 | - | expression tag |
| Met 0 | - | expression tag |
| Ala 203 | Ser 232 | engineered mutation |
| Ala 271 | Ser 300 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 8 |
| Total formula weight | 314135.3 | |
| Non-Polymers* | Number of molecules | 17 |
| Total formula weight | 2537.9 | |
| All* | Total formula weight | 316673.2 |
