3EUK

Crystal structure of MukE-MukF(residues 292-443)-MukB(head domain)-ATPgammaS complex, asymmetric dimer

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, D, E (A, C, F, H)	Chromosome partition protein mukB, Linker	polymer	483	54102.8	4	UniProt (Q7VL96) Pfam (PF04310) Pfam (PF13558)	Haemophilus ducreyi (strain 35000HP / ATCC 700724)	Structural maintenance of chromosome-related protein
2	C, F (E, J)	Chromosome partition protein mukF	polymer	152	17334.5	2	UniProt (Q7VL94) Pfam (PF17193)	Haemophilus ducreyi
3	G, H (L, M)	Chromosome partition protein mukE	polymer	238	26815.5	2	UniProt (Q7VL95) Pfam (PF21980)	Haemophilus ducreyi
4	I, L, M, O (A, C, F, H)	PHOSPHOTHIOPHOSPHORIC ACID-ADENYLATE ESTER	non-polymer		523.2	4	Chemie (AGS)
5	J, K, N, P (A, F, H)	MAGNESIUM ION	non-polymer		24.3	4	Chemie (MG)

Sequence modifications

A, C, F, H: 33 - 271 (UniProt: Q7VL96)

PDB	External Database	Details
Gly 30	-	expression tag
His 31	-	expression tag
Met 32	-	expression tag
Thr 160	Ala 160	SEE REMARK 999

A, C, F, H: 1256 - 1262 (PDB: 3EUK)
A, C, F, H: 1263 - 1496 (UniProt: Q7VL96)

PDB	External Database	Details
Ile 1347	Val 1347	SEE REMARK 999
Tyr 1383	Asp 1383	SEE REMARK 999
Gln 1435	Glu 1435	engineered mutation
Arg 1470	His 1470	SEE REMARK 999

E, J: 292 - 443 (UniProt: Q7VL94)

PDB	External Database	Details
Asn 372	Asp 372	SEE REMARK 999
Gln 384	Arg 384	SEE REMARK 999

L, M: 1 - 238 (UniProt: Q7VL95)

PDB	External Database	Details
Ala 219	Glu 219	SEE REMARK 999

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	304711.3
Non-Polymers*	Number of molecules	8
	Total formula weight	2190.2
All*	Total formula weight	306901.5

*Water molecules are not included.