3ERC
Crystal structure of the heterodimeric vaccinia virus mRNA polyadenylate polymerase with three fragments of RNA and 3'-deoxy ATP
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, B | Cap-specific mRNA (nucleoside-2'-O-)-methyltransferase | polymer | 297 | 34589.0 | 2 | UniProt (P07617) Pfam (PF01358) In PDB | vaccinia virus WR | Poly(A) polymerase regulatory subunit, Poly(A) polymerase small subunit, PAP-S, VP39 |
2 | C, D | Poly(A) polymerase catalytic subunit | polymer | 479 | 55574.1 | 2 | UniProt (P23371) Pfam (PF12630) Pfam (PF03296) Pfam (PF12629) In PDB | vaccinia virus WR | Poly(A) polymerase large subunit, PAP-L, VP55 |
3 | E, F | RNA/DNA chimera (5'-D(CP*)R(UP*UP*)D(CP*C)-3') | polymer | 5 | 1434.9 | 2 | |||
4 | G | RNA/DNA chimera (5'-D(CP*)R(UP*UP*)-D(C)-3') | polymer | 4 | 1145.7 | 1 | |||
5 | I | RNA/DNA chimera (5'-D(CP*CP*)R(UP*UP*)D(C)-3') | polymer | 5 | 1434.9 | 1 | |||
6 | C, D | URIDINE-5'-MONOPHOSPHATE | non-polymer | 324.2 | 2 | Chemie (U) | |||
7 | C, D | 3'-DEOXYADENOSINE-5'-TRIPHOSPHATE | non-polymer | 491.2 | 2 | Chemie (3AT) | |||
8 | C, D | CALCIUM ION | non-polymer | 40.1 | 4 | Chemie (CA) | |||
9 | water | water | 18.0 | 1 | Chemie (HOH) |
Sequence modifications
A, B: 1 - 297 (UniProt: P07617)
C, D: 1 - 479 (UniProt: P23371)
PDB | External Database | Details |
---|---|---|
Ala 140 | Arg 140 | engineered mutation |
Ala 142 | Lys 142 | engineered mutation |
Ala 143 | Arg 143 | engineered mutation |
PDB | External Database | Details |
---|---|---|
Ser 36 | Leu 36 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 8 |
Total formula weight | 185776.7 | |
Non-Polymers* | Number of molecules | 8 |
Total formula weight | 1791.0 | |
All* | Total formula weight | 187567.7 |