3ERC

Crystal structure of the heterodimeric vaccinia virus mRNA polyadenylate polymerase with three fragments of RNA and 3'-deoxy ATP

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Cap-specific mRNA (nucleoside-2'-O-)-methyltransferase	polymer	297	34589.0	2	UniProt (P07617) Pfam (PF01358)	vaccinia virus WR	Poly(A) polymerase regulatory subunit, Poly(A) polymerase small subunit, PAP-S, VP39
2	C, D (C, D)	Poly(A) polymerase catalytic subunit	polymer	479	55574.1	2	UniProt (P23371) Pfam (PF12630) Pfam (PF03296) Pfam (PF12629)	vaccinia virus WR	Poly(A) polymerase large subunit, PAP-L, VP55
3	E, F (E, F)	RNA/DNA chimera (5'-D(CP*)R(UP*UP*)D(CP*C)-3')	polymer	5	1434.9	2
4	G (G)	RNA/DNA chimera (5'-D(CP*)R(UP*UP*)-D(C)-3')	polymer	4	1145.7	1
5	H (I)	RNA/DNA chimera (5'-D(CP*CP*)R(UP*UP*)D(C)-3')	polymer	5	1434.9	1
6	I, M (C, D)	URIDINE-5'-MONOPHOSPHATE	non-polymer		324.2	2	Chemie (U5P)
7	J, N (C, D)	3'-DEOXYADENOSINE-5'-TRIPHOSPHATE	non-polymer		491.2	2	Chemie (3AT)
8	K, L, O, P (C, D)	CALCIUM ION	non-polymer		40.1	4	Chemie (CA)
9	Q (C)	water	water		18.0	1	Chemie (HOH)

Sequence modifications

A, B: 1 - 297 (UniProt: P07617)

PDB	External Database	Details
Ala 140	Arg 140	engineered mutation
Ala 142	Lys 142	engineered mutation
Ala 143	Arg 143	engineered mutation

C, D: 1 - 479 (UniProt: P23371)

PDB	External Database	Details
Ser 36	Leu 36	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	185776.7
Non-Polymers*	Number of molecules	8
	Total formula weight	1791.0
All*	Total formula weight	187567.7

*Water molecules are not included.