3ENM

The structure of the MAP2K MEK6 reveals an autoinhibitory dimer

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	Dual specificity mitogen-activated protein kinase kinase 6	polymer	316	36664.1	4	UniProt (P52564) Pfam (PF00069)	Homo sapiens (Human)	MAP kinase kinase 6, MAPKK 6, MAPK/ERK kinase 6, SAPKK3
2	E, G, I, J (A, B, C, D)	SULFATE ION	non-polymer		96.1	4	Chemie (SO4)
3	F (A)	GLYCEROL	non-polymer		92.1	1	Chemie (GOL)
4	H (B)	1,2-ETHANEDIOL	non-polymer		62.1	1	Chemie (EDO)
5	K, L, M, N (A, B, C, D)	water	water		18.0	325	Chemie (HOH)

Sequence modifications

A, B, C, D: 45 - 332 (UniProt: P52564)

PDB	External Database	Details
Mse 18	-	expression tag
Ser 19	-	expression tag
Tyr 20	-	expression tag
Tyr 21	-	expression tag
His 22	-	expression tag
His 23	-	expression tag
His 24	-	expression tag
His 25	-	expression tag
His 26	-	expression tag
His 27	-	expression tag
Asp 28	-	expression tag
Tyr 29	-	expression tag
Asp 30	-	expression tag
Ile 31	-	expression tag
Pro 32	-	expression tag
Thr 33	-	expression tag
Thr 34	-	expression tag
Glu 35	-	expression tag
Asn 36	-	expression tag
Leu 37	-	expression tag
Tyr 38	-	expression tag
Phe 39	-	expression tag
Gln 40	-	expression tag
Gly 41	-	expression tag
Ala 42	-	expression tag
Mse 43	-	expression tag
Glu 44	-	expression tag
Asp 207	Ser 207	engineered mutation
Asp 211	Thr 211	engineered mutation
Ala 333	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	146656.2
Non-Polymers*	Number of molecules	6
	Total formula weight	538.4
All*	Total formula weight	147194.7

*Water molecules are not included.