3EKJ

Calcium-free GCaMP2 (calcium binding deficient mutant)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Myosin light chain kinase, Green fluorescent protein, Calmodulin chimera	polymer	451	50482.5	1	UniProt (P42212) UniProt (P0DP29) Pfam (PF13499) UniProt (by SIFTS) (P0DP31)	artificial gene
2	B (A)	water	water		18.0	5	Chemie (HOH)

Sequence modifications

A: 1 - 61 (PDB: 3EKJ)
A: 62 - 151 (UniProt: P42212)

PDB	External Database	Details
Ala 76	Val 163	see remark 999
Gly 88	Ser 175	see remark 999
Tyr 93	Asp 180	see remark 999
Lys 119	Ala 206	see remark 999
Leu 144	His 231	see remark 999

A: 160 - 302 (UniProt: P42212)

PDB	External Database	Details
Gly 152	-	linker
Gly 153	-	linker
Thr 154	-	linker
Gly 155	-	linker
Gly 156	-	linker
Ser 157	-	linker
Met 158	-	linker
Val 159	-	linker
Leu 222	Phe 64	see remark 999
Cro 224	Ser 65	chromophore
-	Tyr 66	chromophore
-	Gly 67	chromophore
Ile 251	Val 93	see remark 999
Thr 303	-	linker
Arg 304	-	linker

A: 305 - 451 (UniProt: P0DP29)

PDB	External Database	Details
Gly 329	Thr 27	engineered mutation
Gln 334	Glu 32	engineered mutation
Gly 359	Asp 57	engineered mutation
Gln 370	Glu 68	engineered mutation
Gly 396	Asp 94	engineered mutation
Gln 407	Glu 105	engineered mutation
Gly 432	Asp 130	engineered mutation
Gln 443	Glu 141	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	50482.5
All*	Total formula weight	50482.5

*Water molecules are not included.