3EHU

Crystal structure of the extracellular domain of human corticotropin releasing factor receptor type 1 (CRFR1) in complex with CRF

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	FUSION PROTEIN OF CRFR1 EXTRACELLULAR DOMAIN AND MBP	polymer	476	52605.2	2	UniProt (P0AEX9) UniProt (P34998) Pfam (PF01547) Pfam (PF02793)	Escherichia coli	MMBP, Maltodextrin-binding protein, CRF-R, CRF1, Corticotropin-releasing hormone receptor 1, CRH-R 1
2	C, D (C, D)	Corticoliberin	polymer	21	2309.7	2	UniProt (P06850) Pfam (PF00473)		Corticotropin-releasing hormone, Corticotropin-releasing factor, CRF
3	E, F (E, F)	alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose	branched		342.3	2	In PDB BIRD (PRD_900001) GlyTouCan (G07411ON)		alpha-maltose
4	G, J (A, B)	CALCIUM ION	non-polymer		40.1	2	Chemie (CA)
5	H, K (A, B)	2-[BIS-(2-HYDROXY-ETHYL)-AMINO]-2-HYDROXYMETHYL-PROPANE-1,3-DIOL	non-polymer		209.2	2	Chemie (BTB)
6	I, L (A, B)	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	2	Chemie (PEG)
7	M, N, O, P (A, B, C, D)	water	water		18.0	450	Chemie (HOH)

Sequence modifications

A, B: -349 - 17 (UniProt: P0AEX9)

PDB	External Database	Details
Met -350	-	initiating methionine
Glu -25	Ala 350	engineered mutation
Asn 18	-	linker
Ala 19	-	linker
Ala 20	-	linker
Ala 21	-	linker
Glu 22	-	linker
Phe 23	-	linker
His 120	-	expression tag
His 121	-	expression tag
His 122	-	expression tag
His 123	-	expression tag
His 124	-	expression tag
His 125	-	expression tag

A, B: 24 - 119 (UniProt: P34998)

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	109829.7
Branched	Number of molecules	2
	Total formula weight	684.6
Non-Polymers*	Number of molecules	6
	Total formula weight	710.9
All*	Total formula weight	111225.2

*Water molecules are not included.