3EH8
Crystal structure of Y2 I-AniI variant (F13Y/S111Y)/DNA complex with calcium
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C (A, D, G) | Intron-encoded DNA endonuclease I-AniI | polymer | 254 | 29611.5 | 3 | UniProt (P03880) Pfam (PF00961) | Emericella nidulans (Aspergillus nidulans) | mRNA maturase bI1, COB intron protein, Truncated non-functional cytochrome b, DNA endonuclease/RNA maturase I-AniI |
| 2 | D, F, H (B, E, H) | 31-MER | polymer | 31 | 9609.2 | 3 | |||
| 3 | E, G, I (C, F, I) | 31-MER | polymer | 31 | 9458.1 | 3 | |||
| 4 | J, K, L, M, N... (A, D, G) | CALCIUM ION | non-polymer | 40.1 | 6 | Chemie (CA) | |||
| 5 | P, Q, R, S, T... (A, D, G, B, C...) | water | water | 18.0 | 67 | Chemie (HOH) |
Sequence modifications
A, D, G: 3 - 254 (UniProt: P03880)
| PDB | External Database | Details |
|---|---|---|
| Gly 1 | - | expression tag |
| Ser 2 | - | expression tag |
| Tyr 13 | Phe 247 | engineered mutation |
| Arg 61 | Ile 295 | conflict |
| Lys 80 | Phe 314 | engineered mutation |
| Tyr 111 | Ser 345 | engineered mutation |
| Lys 232 | Leu 466 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 9 |
| Total formula weight | 146036.2 | |
| Non-Polymers* | Number of molecules | 6 |
| Total formula weight | 240.5 | |
| All* | Total formula weight | 146276.7 |
