3EGW
The crystal structure of the NarGHI mutant NarH - C16A
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Respiratory nitrate reductase 1 alpha chain | polymer | 1244 | 140178.4 | 1 | UniProt (P09152) Pfam (PF14710) Pfam (PF00384) Pfam (PF01568) | Escherichia coli | Nitrate reductase A subunit alpha, Quinol-nitrate oxidoreductase alpha subunit |
| 2 | B (B) | Respiratory nitrate reductase 1 beta chain | polymer | 509 | 57775.9 | 1 | UniProt (P11349) Pfam (PF13247) Pfam (PF14711) | Escherichia coli | Nitrate reductase A subunit beta, Quinol-nitrate oxidoreductase subunit beta |
| 3 | C (C) | Respiratory nitrate reductase 1 gamma chain | polymer | 225 | 25402.2 | 1 | UniProt (P11350) Pfam (PF02665) | Escherichia coli | Nitrate reductase A subunit gamma, Quinol-nitrate oxidoreductase gamma subunit, Cytochrome B-NR |
| 4 | D (A) | PHOSPHORIC ACID 4-(2-AMINO-4-OXO-3,4,5,6,-TETRAHYDRO-PTERIDIN-6-YL)-2-HYDROXY-3,4-DIMERCAPTO-BUT-3-EN-YL ESTER GUANYLATE ESTER | non-polymer | 740.6 | 1 | Chemie (MD1) | |||
| 5 | E (A) | 2-AMINO-5,6-DIMERCAPTO-7-METHYL-3,7,8A,9-TETRAHYDRO-8-OXA-1,3,9,10-TETRAAZA-ANTHRACEN-4-ONE GUANOSINE DINUCLEOTIDE | non-polymer | 740.6 | 1 | Chemie (MGD) | |||
| 6 | F (A) | MOLYBDENUM(VI) ION | non-polymer | 95.9 | 1 | Chemie (6MO) | |||
| 7 | G, I, J (A, B) | IRON/SULFUR CLUSTER | non-polymer | 351.6 | 3 | Chemie (SF4) | |||
| 8 | H, K (B) | FE3-S4 CLUSTER | non-polymer | 295.8 | 2 | Chemie (F3S) | |||
| 9 | L (B) | 1,2-DIACYL-GLYCEROL-3-SN-PHOSPHATE | non-polymer | 705.0 | 1 | Chemie (3PH) | |||
| 10 | M, N (C) | PROTOPORPHYRIN IX CONTAINING FE | non-polymer | 616.5 | 2 | Chemie (HEM) | |||
| 11 | O (C) | (1S)-2-{[{[(2S)-2,3-DIHYDROXYPROPYL]OXY}(HYDROXY)PHOSPHORYL]OXY}-1-[(PENTANOYLOXY)METHYL]ETHYL OCTANOATE | non-polymer | 455.5 | 1 | Chemie (AGA) | |||
| 12 | P, Q, R (A, B, C) | water | water | 18.0 | 1526 | Chemie (HOH) |
Sequence modifications
A: 1 - 1244 (UniProt: P09152)
B: 1 - 509 (UniProt: P11349)
C: 1 - 225 (UniProt: P11350)
| PDB | External Database | Details |
|---|---|---|
| Ala 10 | Phe 11 | conflict |
| Ala 362 | Asp 363 | conflict |
| PDB | External Database | Details |
|---|---|---|
| Ala 16 | Cys 16 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Ala 77 | Tyr 77 | conflict |
| Ala 78 | Glu 78 | conflict |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 3 |
| Total formula weight | 223356.5 | |
| Non-Polymers* | Number of molecules | 12 |
| Total formula weight | 5617.0 | |
| All* | Total formula weight | 228973.5 |
