3EGH

Crystal structure of a complex between Protein Phosphatase 1 alpha (PP1), the PP1 binding and PDZ domains of Spinophilin and the small natural molecular toxin Nodularin-R

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Serine/threonine-protein phosphatase PP1-alpha catalytic subunit	polymer	329	37349.8	2	UniProt (P62136) Pfam (PF16891) Pfam (PF00149) In PDB	Homo sapiens (Human)	PP-1A
2	C, D	Spinophilin	polymer	170	18571.6	2	UniProt (O35274) Pfam (PF17817) Pfam (PF00595) In PDB	Rattus norvegicus (Rat)	Neurabin-II, Neurabin-2, Protein phosphatase 1 regulatory subunit 9B, Neural tissue-specific F-actin-binding protein II, p130, PP1bp134
3	E, F	nodularin R	polymer	5	844.0	2	NOR (NOR00279) BIRD (PRD_000214)	Nodularia spumigena
4	A, C	GLYCEROL	non-polymer		92.1	4	Chemie (GOL)
5	A, B	MANGANESE (II) ION	non-polymer		54.9	4	Chemie (MN)
6		water	water		18.0	481	Chemie (HOH)

Sequence modifications

A, B: 7 - 330 (UniProt: P62136)

PDB	External Database	Details
Gly 2	-	expression tag
His 3	-	expression tag
Met 4	-	expression tag
Gly 5	-	expression tag
Ser 6	-	expression tag

C, D: 417 - 583 (UniProt: O35274)

PDB	External Database	Details
Gly 414	-	expression tag
Ser 415	-	expression tag
Met 416	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	113530.8
Non-Polymers*	Number of molecules	8
	Total formula weight	588.1
All*	Total formula weight	114118.9

*Water molecules are not included.