3EGG

Crystal structure of a complex between Protein Phosphatase 1 alpha (PP1) and the PP1 binding and PDZ domains of Spinophilin

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Serine/threonine-protein phosphatase PP1-alpha catalytic subunit	polymer	329	37349.8	2	UniProt (P62136) Pfam (PF16891) Pfam (PF00149)	Homo sapiens (Human)	PP-1A
2	C, D (C, D)	Spinophilin	polymer	170	18622.6	2	UniProt (O35274) Pfam (PF17817) Pfam (PF00595)	Rattus norvegicus (Rat)	Neurabin-II, Neurabin-2, Protein phosphatase 1 regulatory subunit 9B, Neural tissue-specific F-actin-binding protein II, p130, PP1bp134
3	E, F, G, K, L... (A, B, C, D)	GLYCEROL	non-polymer		92.1	7	Chemie (GOL)
4	H, I, M, N (A, B)	MANGANESE (II) ION	non-polymer		54.9	4	Chemie (MN)
5	J, O, P (A, B)	2-(N-MORPHOLINO)-ETHANESULFONIC ACID	non-polymer		195.2	3	Chemie (MES)
6	S, T, U, V (A, B, C, D)	water	water		18.0	521	Chemie (HOH)

Sequence modifications

A, B: 7 - 330 (UniProt: P62136)

PDB	External Database	Details
Gly -4	-	expression tag
His -3	-	expression tag
Met -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag

C, D: 417 - 583 (UniProt: O35274)

PDB	External Database	Details
Gly -2	-	expression tag
His -1	-	expression tag
Met 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	111945.0
Non-Polymers*	Number of molecules	14
	Total formula weight	1450.1
All*	Total formula weight	113395.1

*Water molecules are not included.