3ECP
Crystal Structure Of Tn5 Transposase Complexed With 5' Phosphorylated Transposon End DNA
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A | Tn5 transposase | polymer | 477 | 53367.0 | 1 | UniProt (Q08JA5) Pfam (PF14706) Pfam (PF01609) UniProt (by SIFTS) (Q46731) In PDB | Escherichia coli | |
| 2 | B | DNA transferred strand | polymer | 20 | 6213.0 | 1 | |||
| 3 | C | DNA non-transferred strand | polymer | 20 | 6052.9 | 1 | |||
| 4 | A | GLYCEROL | non-polymer | 92.1 | 3 | Chemie (GOL) | |||
| 5 | water | water | 18.0 | 120 | Chemie (HOH) |
Sequence modifications
A: 1 - 476 (UniProt: Q08JA5)
| PDB | External Database | Details |
|---|---|---|
| Lys 54 | Glu 58 | engineered mutation |
| Ala 56 | Met 60 | engineered mutation |
| Pro 372 | Leu 376 | engineered mutation |
| Gly 477 | - | cloning artifact |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 3 |
| Total formula weight | 65633.0 | |
| Non-Polymers* | Number of molecules | 3 |
| Total formula weight | 276.3 | |
| All* | Total formula weight | 65909.3 |
