3EA5
Kap95p Binding Induces the Switch Loops of RanGDP to adopt the GTP-bound Conformation: Implications for Nuclear Import Complex Assembly Dynamics
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, C (A, C) | GTP-binding nuclear protein Ran | polymer | 216 | 24488.2 | 2 | UniProt (P62826) Pfam (PF00071) | Homo sapiens (human) | GTPase Ran, Ras-related nuclear protein, Ras-like protein TC4, Androgen receptor-associated protein 24 |
| 2 | B, D (B, D) | Importin subunit beta-1 | polymer | 861 | 94859.3 | 2 | UniProt (Q06142) Pfam (PF03810) Pfam (PF02985) | Saccharomyces cerevisiae (brewer's yeast lager beer yeast yeast) | Karyopherin subunit beta-1, Karyopherin-95, Importin-95 |
| 3 | E, H (A, C) | GUANOSINE-5'-DIPHOSPHATE | non-polymer | 443.2 | 2 | Chemie (GDP) | |||
| 4 | F, G, I, J (A, C) | MAGNESIUM ION | non-polymer | 24.3 | 4 | Chemie (MG) | |||
| 5 | K, L, M, N (A, B, C, D) | water | water | 18.0 | 364 | Chemie (HOH) |
Sequence modifications
A, C: 1 - 216 (UniProt: P62826)
B, D: 1 - 861 (UniProt: Q06142)
| PDB | External Database | Details |
|---|---|---|
| Cys 181 | Ala 181 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Lys 254 | Leu 254 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 238694.9 | |
| Non-Polymers* | Number of molecules | 6 |
| Total formula weight | 983.6 | |
| All* | Total formula weight | 239678.5 |
