3EA3

Crystal Structure of the Y246S/Y247S/Y248S/Y251S Mutant of Phosphatidylinositol-Specific Phospholipase C from Bacillus Thuringiensis

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	1-phosphatidylinositol phosphodiesterase	polymer	298	34245.2	2	UniProt (P08954) Pfam (PF00388)	Bacillus thuringiensis	Phosphatidylinositol diacylglycerol-lyase, Phosphatidylinositol-specific phospholipase C, PI-PLC
2	C, D, E, F, G (A, B)	MANGANESE (II) ION	non-polymer		54.9	5	Chemie (MN)
3	H, I (A, B)	water	water		18.0	525	Chemie (HOH)

Sequence modifications

A, B: 1 - 298 (UniProt: P08954)

PDB	External Database	Details
Ser 246	Tyr 277	engineered mutation
Ser 247	Tyr 278	engineered mutation
Ser 248	Tyr 279	engineered mutation
Ser 251	Tyr 282	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	68490.3
Non-Polymers*	Number of molecules	5
	Total formula weight	274.7
All*	Total formula weight	68765.0

*Water molecules are not included.