3EA1

Crystal Structure of the Y247S/Y251S Mutant of Phosphatidylinositol-Specific Phospholipase C from Bacillus Thuringiensis

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	1-phosphatidylinositol phosphodiesterase	polymer	298	34397.4	2	UniProt (P08954) Pfam (PF00388)	Bacillus thuringiensis	Phosphatidylinositol diacylglycerol-lyase, Phosphatidylinositol-specific phospholipase C, PI-PLC
2	C, D, E, F, G... (A, B)	ZINC ION	non-polymer		65.4	6	Chemie (ZN)
3	I, J (A, B)	water	water		18.0	622	Chemie (HOH)

Sequence modifications

A, B: 1 - 298 (UniProt: P08954)

PDB	External Database	Details
Ser 247	Tyr 278	engineered mutation
Ser 251	Tyr 282	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	68794.7
Non-Polymers*	Number of molecules	6
	Total formula weight	392.5
All*	Total formula weight	69187.2

*Water molecules are not included.