3E50

Crystal structure of human insulin degrading enzyme in complex with transforming growth factor-alpha

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Insulin-degrading enzyme	polymer	990	114715.1	2	UniProt (P14735) Pfam (PF00675) Pfam (PF05193) Pfam (PF16187)	Homo sapiens (Human)	Insulin protease, Insulinase, Insulysin
2	C, D (C, D)	Protransforming growth factor alpha	polymer	50	5560.2	2	UniProt (P01135)	Homo sapiens (Human)	Transforming growth factor alpha, TGF-alpha, EGF-like TGF, ETGF, TGF type 1
3	E, F (A, D)	ZINC ION	non-polymer		65.4	2	Chemie (ZN)
4	G, H (A, B)	water	water		18.0	568	Chemie (HOH)

Sequence modifications

A, B: 42 - 1019 (UniProt: P14735)

PDB	External Database	Details
Met 30	-	expression tag
His 31	-	expression tag
His 32	-	expression tag
His 33	-	expression tag
His 34	-	expression tag
His 35	-	expression tag
His 36	-	expression tag
Ala 37	-	expression tag
Ala 38	-	expression tag
Gly 39	-	expression tag
Ile 40	-	expression tag
Pro 41	-	expression tag
Gln 111	Glu 111	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	240550.8
Non-Polymers*	Number of molecules	2
	Total formula weight	130.8
All*	Total formula weight	240681.6

*Water molecules are not included.