3DYO
E. coli (lacZ) beta-galactosidase (H418N) in complex with IPTG
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C, D (A, B, C, D) | Beta-galactosidase | polymer | 1023 | 116482.2 | 4 | UniProt (P00722) Pfam (PF02837) Pfam (PF00703) Pfam (PF02836) Pfam (PF16353) Pfam (PF02929) | Escherichia coli K12 | |
| 2 | AC, BC, E, F, G... (C, A, D, B) | MAGNESIUM ION | non-polymer | 24.3 | 14 | Chemie (MG) | |||
| 3 | CC, DC, EC, FC, GC... (C, A, D, B) | SODIUM ION | non-polymer | 23.0 | 18 | Chemie (NA) | |||
| 4 | HC, HD, IC, M, MA... (C, A, D, B) | 1-methylethyl 1-thio-beta-D-galactopyranoside | non-polymer | 238.3 | 12 | Chemie (IPT) | |||
| 5 | AA, AB, AD, AE, BA... (A, B, C, D) | DIMETHYL SULFOXIDE | non-polymer | 78.1 | 97 | Chemie (DMS) | |||
| 6 | PE, QE, RE, SE (A, B, C, D) | water | water | 18.0 | 3728 | Chemie (HOH) |
Sequence modifications
A, B, C, D: 9 - 1023 (UniProt: P00722)
| PDB | External Database | Details |
|---|---|---|
| Gly 1 | - | expression tag |
| Ser 2 | - | expression tag |
| His 3 | - | expression tag |
| Met 4 | - | expression tag |
| Leu 5 | - | expression tag |
| Glu 6 | - | expression tag |
| Asp 7 | - | expression tag |
| Pro 8 | - | expression tag |
| Asn 418 | His 419 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 465928.9 | |
| Non-Polymers* | Number of molecules | 141 |
| Total formula weight | 11192.6 | |
| All* | Total formula weight | 477121.5 |
