3DYO
E. coli (lacZ) beta-galactosidase (H418N) in complex with IPTG
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C, D (A, B, C, D) | Beta-galactosidase | polymer | 1023 | 116482.2 | 4 | UniProt (P00722) Pfam (PF02837) Pfam (PF00703) Pfam (PF02836) Pfam (PF16353) Pfam (PF02929) | Escherichia coli K12 | |
| 2 | AC, BC, E, F, G... (C, A, D, B) | MAGNESIUM ION | non-polymer | 24.3 | 14 | Chemie (MG) PubChem (888) | |||
| 3 | CC, DC, EC, FC, GC... (C, A, D, B) | SODIUM ION | non-polymer | 23.0 | 18 | Chemie (NA) PubChem (923) | |||
| 4 | HC, HD, IC, M, MA... (C, A, D, B) | 1-methylethyl 1-thio-beta-D-galactopyranoside | non-polymer | 238.3 | 12 | Chemie (IPT) PubChem (16760528) PubChem (2802070) PubChem (22819629) PubChem (22836579) PubChem (24802248) PubChem (25113457) PubChem (25322847) PubChem (9711) PubChem (26470596) PubChem (26470897) PubChem (26470899) PubChem (38989032) PubChem (40489056) PubChem (16217509) PubChem (53694108) PubChem (69133132) PubChem (70877296) PubChem (552632) PubChem (656894) PubChem (853436) PubChem (92223493) PubChem (92843742) PubChem (92843743) PubChem (95387455) PubChem (95387456) PubChem (6928325) PubChem (134694195) PubChem (122599329) PubChem (129353964) PubChem (131676754) PubChem (140882545) PubChem (140882549) PubChem (172897862) PubChem (175675668) | |||
| 5 | AA, AB, AD, AE, BA... (A, B, C, D) | DIMETHYL SULFOXIDE | non-polymer | 78.1 | 97 | Chemie (DMS) PubChem (679) | |||
| 6 | PE, QE, RE, SE (A, B, C, D) | water | water | 18.0 | 3728 | Chemie (HOH) |
Sequence modifications
A, B, C, D: 9 - 1023 (UniProt: P00722)
| PDB | External Database | Details |
|---|---|---|
| Gly 1 | - | expression tag |
| Ser 2 | - | expression tag |
| His 3 | - | expression tag |
| Met 4 | - | expression tag |
| Leu 5 | - | expression tag |
| Glu 6 | - | expression tag |
| Asp 7 | - | expression tag |
| Pro 8 | - | expression tag |
| Asn 418 | His 419 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 465928.9 | |
| Non-Polymers* | Number of molecules | 141 |
| Total formula weight | 11192.6 | |
| All* | Total formula weight | 477121.5 |
