3DVG

Crystal structure of K63-specific fab Apu.3A8 bound to K63-linked di-ubiquitin

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Human IgG1 fab fragment light chain	polymer	217	23731.3	1	Pfam (PF07686) Pfam (PF07654) UniProt (by SIFTS) (Q6PIL8) In PDB	Homo sapiens (Human)
2	B	Human IgG1 fab fragment heavy chain	polymer	230	24590.5	1		Homo sapiens (Human)
3	X	Ubiquitin D77	polymer	80	8974.2	1	UniProt (P62988) Pfam (PF00240) UniProt (by SIFTS) (P0CG48) In PDB	Homo sapiens (Human)
4	Y	Ubiquitin	polymer	79	8887.1	1	UniProt (P62988) Pfam (PF00240) UniProt (by SIFTS) (P0CG48) In PDB	Homo sapiens (Human)
5		water	water		18.0	25	Chemie (HOH)

Sequence modifications

X: 1 - 76 (UniProt: P62988)

PDB	External Database	Details
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
Asp 77	-	engineered mutation

Y: 1 - 76 (UniProt: P62988)

PDB	External Database	Details
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
Arg 63	Lys 63	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	66183.1
All*	Total formula weight	66183.1

*Water molecules are not included.