3DU3

E(L212)A, D(L213)A, A(M249)Y triple mutant structure of photosynthetic reaction center

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (L)	Reaction center protein L chain	polymer	281	31244.3	1	UniProt (P0C0Y8) Pfam (PF00124)	Rhodobacter sphaeroides (Rhodopseudomonas sphaeroides)	Photosynthetic reaction center L subunit
2	B (M)	Reaction center protein M chain	polymer	314	35457.7	1	UniProt (P0C0Y9) Pfam (PF00124)	Rhodobacter sphaeroides (Rhodopseudomonas sphaeroides)	Photosynthetic reaction center M subunit
3	C (H)	Reaction center protein H chain	polymer	260	28066.3	1	UniProt (P0C0Y7) Pfam (PF03967) Pfam (PF05239)	Rhodobacter sphaeroides (Rhodopseudomonas sphaeroides)	Photosynthetic reaction center H subunit
4	D, E, H, I (L, M)	BACTERIOCHLOROPHYLL A	non-polymer		911.5	4	Chemie (BCL)
5	F, J (L, M)	BACTERIOPHEOPHYTIN A	non-polymer		889.2	2	Chemie (BPH)
6	G, L (L, M)	UBIQUINONE-10	non-polymer		863.3	2	Chemie (U10)
7	K (M)	FE (III) ION	non-polymer		55.8	1	Chemie (FE)
8	M (M)	SPEROIDENONE	non-polymer		595.0	1	Chemie (SPN)
9	N (M)	CARDIOLIPIN	non-polymer		1464.0	1	Chemie (CDL)
10	O, P, Q (M, H)	LAURYL DIMETHYLAMINE-N-OXIDE	non-polymer		229.4	3	Chemie (LDA)
11	R, S, T (L, M, H)	water	water		18.0	166	Chemie (HOH)

Sequence modifications

L: 1 - 281 (UniProt: P0C0Y8)

PDB	External Database	Details
Ala 212	Glu 213	engineered mutation
Ala 213	Asp 214	engineered mutation

M: 1 - 307 (UniProt: P0C0Y9)

PDB	External Database	Details
Tyr 249	Ala 250	engineered mutation
His 308	-	expression tag
His 309	-	expression tag
His 310	-	expression tag
His 311	-	expression tag
His 312	-	expression tag
His 313	-	expression tag
His 314	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	94768.3
Non-Polymers*	Number of molecules	14
	Total formula weight	9954.2
All*	Total formula weight	104722.5

*Water molecules are not included.