3DGI
Crystal structure of F87A/T268A mutant of CYP BM3
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | Bifunctional P-450/NADPH-P450 reductase | polymer | 461 | 52892.2 | 2 | UniProt (P14779) Pfam (PF00067) | Bacillus megaterium | Cytochrome P450(BM-3), P450BM-3 [Full length protein includes: Cytochrome P450 102 (EC 1.14.14.1) and NADPH-cytochrome P450 reductase (EC 1.6.2.4)] |
| 2 | C, E (A, B) | PROTOPORPHYRIN IX CONTAINING FE | non-polymer | 616.5 | 2 | Chemie (HEM) | |||
| 3 | D, F (A, B) | DIMETHYL SULFOXIDE | non-polymer | 78.1 | 2 | Chemie (DMS) | |||
| 4 | G, H (A, B) | water | water | 18.0 | 508 | Chemie (HOH) |
Sequence modifications
A, B: 1 - 455 (UniProt: P14779)
| PDB | External Database | Details |
|---|---|---|
| Ala 87 | Phe 88 | engineered mutation |
| Ala 268 | Thr 269 | engineered mutation |
| His 456 | - | expression tag |
| His 457 | - | expression tag |
| His 458 | - | expression tag |
| His 459 | - | expression tag |
| His 460 | - | expression tag |
| His 461 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 105784.4 | |
| Non-Polymers* | Number of molecules | 4 |
| Total formula weight | 1389.2 | |
| All* | Total formula weight | 107173.7 |
