3DCO

Drosophila NOD (3DC4) and Bovine Tubulin (1JFF) Docked into the 11-Angstrom Cryo-EM Map of Nucleotide-Free NOD Complexed to the Microtubule

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (N)	Kinesin-like protein Nod	polymer	344	38305.6	1	UniProt (P18105) Pfam (PF00225)	Drosophila melanogaster (Fruit fly)
2	B (A)	Bovine Alpha Tubulin	polymer	451	50107.2	1	Pfam (PF00091) Pfam (PF03953) UniProt (by SIFTS) (P81947)	Bos taurus
3	C (B)	Bovine Beta Tubulin	polymer	445	49907.8	1	Pfam (PF00091) Pfam (PF03953) UniProt (by SIFTS) (Q6B856)	Bos taurus
4	D, F (N, A)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
5	E (N)	ADENOSINE-5'-DIPHOSPHATE	non-polymer		427.2	1	Chemie (ADP)
6	G (A)	ZINC ION	non-polymer		65.4	1	Chemie (ZN)
7	H (B)	GUANOSINE-5'-TRIPHOSPHATE	non-polymer		523.2	1	Chemie (GTP)
8	I (B)	GUANOSINE-5'-DIPHOSPHATE	non-polymer		443.2	1	Chemie (GDP)
9	J (B)	TAXOL	non-polymer		853.9	1	Chemie (TA1)

Sequence modifications

N: 1 - 318 (UniProt: P18105)

PDB	External Database	Details
Met -14	-	expression tag
Ala -13	-	expression tag
Ser -12	-	expression tag
Asp -11	-	expression tag
Tyr -10	-	expression tag
Lys -9	-	expression tag
Asp -8	-	expression tag
Asp -7	-	expression tag
Asp -6	-	expression tag
Asp -5	-	expression tag
Lys -4	-	expression tag
Arg -3	-	expression tag
Arg -2	-	expression tag
Arg -1	-	expression tag
Gly 0	-	expression tag
Ala 319	-	expression tag
Ala 320	-	expression tag
Ala 321	-	expression tag
Leu 322	-	expression tag
Glu 323	-	expression tag
His 324	-	expression tag
His 325	-	expression tag
His 326	-	expression tag
His 327	-	expression tag
His 328	-	expression tag
His 329	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	138320.6
Non-Polymers*	Number of molecules	7
	Total formula weight	2361.5
All*	Total formula weight	140682.1

*Water molecules are not included.