3DBL
Structural Dissection of a Gating Mechanism Preventing Misactivation of Ubiquitin by NEDD8's E1 (APPBP1-UBA3Arg190wt-NEDD8Ala72Gln)
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, C, E, G | NEDD8-activating enzyme E1 regulatory subunit | polymer | 531 | 59973.8 | 4 | UniProt (Q13564) Pfam (PF00899) In PDB | Homo sapiens (Human) | Amyloid protein-binding protein 1, Amyloid beta protein-binding protein 1, 59 kDa, APP-BP1, Proto-oncogene protein 1 |
2 | B, D, F, H | NEDD8-activating enzyme E1 catalytic subunit | polymer | 434 | 48767.8 | 4 | UniProt (Q8TBC4) Pfam (PF00899) Pfam (PF08825) In PDB | Homo sapiens (Human) | Ubiquitin- like modifier-activating enzyme 3, Ubiquitin-activating enzyme 3, NEDD8-activating enzyme E1C, Ubiquitin-activating enzyme E1C |
3 | I, J, K, L | NEDD8 | polymer | 88 | 9692.1 | 4 | UniProt (Q15843) Pfam (PF00240) In PDB | Homo sapiens (Human) | Ubiquitin-like protein Nedd8, Neddylin |
4 | B, D, F, H | ZINC ION | non-polymer | 65.4 | 4 | Chemie (ZN) |
Sequence modifications
A, C, E, G: 1 - 534 (UniProt: Q13564)
B, D, F, H: 12 - 442 (UniProt: Q8TBC4)
I, J, K, L: 101 - 176 (UniProt: Q15843)
PDB | External Database | Details |
---|---|---|
Gly -1 | - | expression tag |
Ser 0 | - | expression tag |
- | Asn 254 | deletion |
- | Glu 255 | deletion |
- | Asn 256 | deletion |
- | Gly 257 | deletion |
- | Ala 258 | deletion |
PDB | External Database | Details |
---|---|---|
Met 9 | - | expression tag |
Lys 10 | - | expression tag |
Leu 11 | - | expression tag |
Ala 216 | Cys 237 | engineered mutation |
PDB | External Database | Details |
---|---|---|
Gly 89 | - | expression tag |
Ser 90 | - | expression tag |
Arg 91 | - | expression tag |
Arg 92 | - | expression tag |
Ala 93 | - | expression tag |
Ser 94 | - | expression tag |
Val 95 | - | expression tag |
Gly 96 | - | expression tag |
Ser 97 | - | expression tag |
Gly 98 | - | expression tag |
Gly 99 | - | expression tag |
Ser 100 | - | expression tag |
Gln 172 | Ala 72 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 12 |
Total formula weight | 473734.7 | |
Non-Polymers* | Number of molecules | 4 |
Total formula weight | 261.6 | |
All* | Total formula weight | 473996.3 |