3D71

Crystal structure of E253Q BMRR bound to 22 base pair promoter site

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	M	BMR promoter DNA	polymer	24	7362.7	1		synthetic construct
2	A	Multidrug-efflux transporter 1 regulator	polymer	284	33477.2	1	UniProt (P39075) Pfam (PF00376) Pfam (PF06445) In PDB	Bacillus subtilis
3	A	ZINC ION	non-polymer		65.4	1	Chemie (ZN)
4	A	CITRATE ANION	non-polymer		189.1	1	Chemie (FLC)
5	A	S-1,2-PROPANEDIOL	non-polymer		76.1	3	Chemie (PGO)
6	A	TRIFLUOROETHANOL	non-polymer		100.0	1	Chemie (ETF)
7	A	IMIDAZOLE	non-polymer		69.1	5	Chemie (IMD)
8		water	water		18.0	55	Chemie (HOH)

Sequence modifications

A: 1 - 278 (UniProt: P39075)

PDB	External Database	Details
Gln 253	Glu 253	engineered mutation
Leu 277	Ala 277	engineered mutation
Asp 278	Glu 278	engineered mutation
His 279	-	expression tag
His 280	-	expression tag
His 281	-	expression tag
His 282	-	expression tag
His 283	-	expression tag
His 284	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	40839.9
Non-Polymers*	Number of molecules	11
	Total formula weight	928.3
All*	Total formula weight	41768.2

*Water molecules are not included.