3D6Y

Crystal structure of R275E mutant of BMRR bound to DNA and berberine

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	B	BMR promoter DNA	polymer	24	7362.7	1		synthetic construct
2	A	Multidrug-efflux transporter 1 regulator	polymer	284	33450.1	1	UniProt (P39075) Pfam (PF00376) Pfam (PF06445) In PDB	Bacillus subtilis
3	A	BERBERINE	non-polymer		336.4	1	Chemie (BER)
4	A	GLYCEROL	non-polymer		92.1	2	Chemie (GOL)
5		water	water		18.0	34	Chemie (HOH)

Sequence modifications

A: 1 - 278 (UniProt: P39075)

PDB	External Database	Details
Glu 275	Arg 275	engineered mutation
Leu 277	Ala 277	engineered mutation
Asp 278	Glu 278	engineered mutation
His 279	-	expression tag
His 280	-	expression tag
His 281	-	expression tag
His 282	-	expression tag
His 283	-	expression tag
His 284	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	40812.8
Non-Polymers*	Number of molecules	3
	Total formula weight	520.5
All*	Total formula weight	41333.4

*Water molecules are not included.