3D6Y
Crystal structure of R275E mutant of BMRR bound to DNA and berberine
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (B) | BMR promoter DNA | polymer | 24 | 7362.7 | 1 | synthetic construct | ||
| 2 | B (A) | Multidrug-efflux transporter 1 regulator | polymer | 284 | 33450.1 | 1 | UniProt (P39075) Pfam (PF00376) Pfam (PF06445) | Bacillus subtilis | |
| 3 | C (A) | BERBERINE | non-polymer | 336.4 | 1 | Chemie (BER) | |||
| 4 | D, E (A) | GLYCEROL | non-polymer | 92.1 | 2 | Chemie (GOL) | |||
| 5 | F (A) | water | water | 18.0 | 34 | Chemie (HOH) |
Sequence modifications
A: 1 - 278 (UniProt: P39075)
| PDB | External Database | Details |
|---|---|---|
| Glu 275 | Arg 275 | engineered mutation |
| Leu 277 | Ala 277 | engineered mutation |
| Asp 278 | Glu 278 | engineered mutation |
| His 279 | - | expression tag |
| His 280 | - | expression tag |
| His 281 | - | expression tag |
| His 282 | - | expression tag |
| His 283 | - | expression tag |
| His 284 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 40812.8 | |
| Non-Polymers* | Number of molecules | 3 |
| Total formula weight | 520.5 | |
| All* | Total formula weight | 41333.4 |
