3D6E

Crystal structure of the engineered 1,3-1,4-beta-glucanase protein from Bacillus licheniformis

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Beta-glucanase	polymer	201	22753.0	2	UniProt (P27051) Pfam (PF00722)	Bacillus licheniformis	Endo-beta-1,3-1,4 glucanase, 1,3-1,4-beta-D-glucan 4-glucanohydrolase, Lichenase
2	C, D (A, B)	CALCIUM ION	non-polymer		40.1	2	Chemie (CA)
3	E, F (A, B)	water	water		18.0	137	Chemie (HOH)

Sequence modifications

A, B: 1 - 201 (UniProt: P27051)

PDB	External Database	Details
-	Ala 50	deletion
-	Asp 51	deletion
-	Gly 52	deletion
-	Tyr 53	deletion
-	Ser 54	deletion
-	Asn 55	deletion
-	Gly 56	deletion
-	Asn 57	deletion
-	Met 58	deletion
-	Phe 59	deletion
-	Asn 60	deletion
-	Cys 61	deletion
-	Thr 62	deletion
Ala 22	Arg 64	engineered mutation
Phe 23	Ala 65	engineered mutation
Asp 24	Asn 66	engineered mutation
His 25	Asn 67	engineered mutation
Gly 48	Cys 90	engineered mutation
Gly 50	Glu 92	engineered mutation
Gln 52	Arg 94	engineered mutation
Ala 77	Ser 119	engineered mutation
Tyr 79	Phe 121	engineered mutation
Ser 81	Tyr 123	engineered mutation
Tyr 167	Met 209	engineered mutation
Ser 169	Asn 211	engineered mutation
Ala 172	Asn 214	engineered mutation
Tyr 190	Ser 232	SEE REMARK 999
Ala 191	Arg 233	SEE REMARK 999
His 192	Ser 234	SEE REMARK 999
Tyr 193	Leu 235	SEE REMARK 999
Asn 194	His 236	SEE REMARK 999

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	45506.1
Non-Polymers*	Number of molecules	2
	Total formula weight	80.2
All*	Total formula weight	45586.2

*Water molecules are not included.