3D4G

ZP-N domain of mammalian sperm receptor ZP3 (crystal form II)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D, E... (A, B, C, D, E...)	Maltose-binding periplasmic protein, LINKER, Zona pellucida protein 3	polymer	481	52948.9	8	UniProt (P0AEX9) UniProt (P10761) Pfam (PF01547) Pfam (PF00100)	Escherichia coli (strain K12)	Maltose-binding protein/ZP3 ZP-N domain chimera, MBP, Zona pellucida glycoprotein ZP3, Sperm receptor, Zona pellucida protein C
2	I, J, K, L, M... (I, J, K, L, M...)	alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose	branched		342.3	8	In PDB BIRD (PRD_900001) GlyTouCan (G07411ON)		alpha-maltose
3	AA, BA, CA, DA, EA... (D, E, F, G, H...)	CALCIUM ION	non-polymer		40.1	24	Chemie (CA)
4	OA, PA, QA, RA, SA... (A, B, C, D, E...)	water	water		18.0	1934	Chemie (HOH)

Sequence modifications

A, B, C, D, E, F, G, H: 2 - 368 (UniProt: P0AEX9)

PDB	External Database	Details
Met 1	-	initiating methionine
Thr 3	Ile 28	engineered mutation
Ala 360	Glu 385	engineered mutation
Ala 363	Lys 388	engineered mutation
Ala 364	Asp 389	engineered mutation
Asn 368	Arg 393	engineered mutation

A, B, C, D, E, F, G, H: 372 - 473 (UniProt: P10761)

PDB	External Database	Details
Leu 474	-	expression tag
Glu 475	-	expression tag
His 476	-	expression tag
His 477	-	expression tag
His 478	-	expression tag
His 479	-	expression tag
His 480	-	expression tag
His 481	-	expression tag

A, B, C, D, E, F, G, H: 369 - 371 (PDB: 3d4G)

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	423591.6
Branched	Number of molecules	8
	Total formula weight	2738.4
Non-Polymers*	Number of molecules	24
	Total formula weight	961.9
All*	Total formula weight	427291.8

*Water molecules are not included.