3D2C

Structure of 4D3, a thermostable mutant of Bacillus subtilis lipase obtained through directed evolution

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D, E...	Lipase	polymer	181	19599.0	12	UniProt (P37957) Pfam (PF01674) In PDB	Bacillus subtilis	Triacylglycerol lipase
2		water	water		18.0	1130	Chemie (HOH)

Sequence modifications

A, B, C, D, E, F, G, H, I, J, K, L: 1 - 181 (UniProt: P37957)

PDB	External Database	Details
Ser 15	Ala 46	engineered mutation
Ser 17	Phe 48	engineered mutation
Glu 20	Ala 51	engineered mutation
Tyr 89	Asn 120	engineered mutation
Asp 111	Gly 142	engineered mutation
Pro 114	Leu 145	engineered mutation
Asp 132	Ala 163	engineered mutation
Met 157	Ile 188	engineered mutation
Tyr 166	Asn 197	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	12
	Total formula weight	235187.5
All*	Total formula weight	235187.5

*Water molecules are not included.