3D2C
Structure of 4D3, a thermostable mutant of Bacillus subtilis lipase obtained through directed evolution
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C, D, E... (A, B, C, D, E...) | Lipase | polymer | 181 | 19599.0 | 12 | UniProt (P37957) Pfam (PF01674) | Bacillus subtilis | Triacylglycerol lipase |
| 2 | M, N, O, P, Q... (A, B, C, D, E...) | water | water | 18.0 | 1130 | Chemie (HOH) |
Sequence modifications
A, B, C, D, E, F, G, H, I, J, K, L: 1 - 181 (UniProt: P37957)
| PDB | External Database | Details |
|---|---|---|
| Ser 15 | Ala 46 | engineered mutation |
| Ser 17 | Phe 48 | engineered mutation |
| Glu 20 | Ala 51 | engineered mutation |
| Tyr 89 | Asn 120 | engineered mutation |
| Asp 111 | Gly 142 | engineered mutation |
| Pro 114 | Leu 145 | engineered mutation |
| Asp 132 | Ala 163 | engineered mutation |
| Met 157 | Ile 188 | engineered mutation |
| Tyr 166 | Asn 197 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 12 |
| Total formula weight | 235187.5 | |
| All* | Total formula weight | 235187.5 |
